The effect of silver and copper nanoparticles on the growth and mycorrhizal colonisation of Scots pine (Pinus sylvestris L.) in a container nursery experiment

doi:10.3832/ifor2855-011

The effect of silver and copper nanoparticles on the growth and mycorrhizal colonisation of Scots pine (Pinus sylvestris L.) in a container nursery experiment^(§)

Marta Aleksandrowicz-Trzcinska⁽¹⁾ , Adam Szaniawski⁽¹⁾, Marcin Studnicki⁽²⁾, Magdalena Bederska-Blaszczyk⁽³⁾, Jacek Olchowik^(1-4), Alexander Urban⁽⁵⁾

iForest - Biogeosciences and Forestry, Volume 11, Issue 5, Pages 690-697 (2018)
doi: https://doi.org/10.3832/ifor2855-011
Published: Oct 23, 2018 - Copyright © 2018 SISEF

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(§): Authorship and acknowledgements of this article were corrected on 10 April 2019 (see 10.3832/ifor2855-011-bis).

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Recent research points to the possibility of nanoparticles being used as fertilisers, growth stimulators, and promoters of plant resistance or pesticides. In this study, we sought to determine the influence of nanoparticles of silver and copper (AgNPs and CuNPs) on growth parameters and spontaneous mycorrhizal colonisation of roots in 2-year-old container-grown seedlings of Scots pine. Foliar applications of nanoparticles were made through two growing seasons, four times a season, at concentrations of 0, 5, 25 and 50 ppm. Comparisons of the ultrastructures characterising the needles, stems and roots of the treated or untreated pines were conducted with transmission electron microscopy (TEM). The deployed CuNPs stimulated mycorrhizal colonisation at all concentrations, although the growth of seedlings was only promoted at a concentration of 25 ppm. Higher concentrations of AgNPs (25 and 50 ppm) inhibited the formation of mycorrhizae, though the lowest concentration (5 ppm) produced an increase in both mycorrhizal colonisation and the dry mass of roots. The species of ectomycorrhizal fungi found were Thelephora terrestris, Suillus bovinus and Sphaerosporella brunnea. The TEM results comparing treated and control (untreated) needles revealed changes in the chloroplasts from lens-shaped to spherical. Furthermore, an increase in the number of plastoglobules and the presence of large osmophilic globules in the cytoplasm associated solely with the needles of pines receiving 50 ppm nanoparticles were observed. In contrast, ultrastructural changes in stems and roots associated with the applications of NPs were not found. Overall, the results indicated that CuNPs and AgNPs could be used as stimulators of growth in general, and mycorrhizal colonisation in particular, among container-grown Scots pines. However, further work is needed to determine their optimal doses and concentrations.

Keywords

Nanoparticles, Ectomycorrhizae, Toxicity, Growth Stimulation

Authors’ address

(1)

Department of Forest Protection and Ecology, Faculty of Forestry, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw (Poland)

(2)

Department of Experimental Design and Bioinformatics, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska 159, Warsaw 02-776 (Poland)

(3)

Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska 159, Warsaw 02-776 (Poland)

(4)

Faculty of Horticulture, Biotechnology and Landscape Architectur, Department of Plant Pathology, Nowoursynowska 159, Warszaw 02-776 (Poland) (since October 2018)

(5)

Department of Botany and Biodiversity Research, Faculty of Life Sciences, University of Vienna, Rennweg 14, Wien A-1030 (Austria)

Corresponding author

Marta Aleksandrowicz-Trzcinska
marta_aleksandrowicz_trzcinska@sggw.pl

Citation

Aleksandrowicz-Trzcinska M, Szaniawski A, Studnicki M, Bederska-Blaszczyk M, Olchowik J, Urban A (2018). The effect of silver and copper nanoparticles on the growth and mycorrhizal colonisation of Scots pine (Pinus sylvestris L.) in a container nursery experiment. iForest 11: 690-697. - doi: 10.3832/ifor2855-011

Academic Editor

Claudia Cocozza

Paper history

Received: May 18, 2018
Accepted: Jul 22, 2018

First online: Oct 23, 2018
Publication Date: Oct 31, 2018
Publication Time: 3.10 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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