iForest - Biogeosciences and Forestry


De novo adventitious root formations in mini-cuttings of Azadirachta indica in response to different rooting media and auxin treatments

Ashok Gehlot (1)   , Rajesh Kumar Gupta (1), Inder Dev Arya (1), Sarita Arya (1), Atul Tripathi (2)

iForest - Biogeosciences and Forestry, Volume 8, Issue 4, Pages 558-564 (2014)
doi: https://doi.org/10.3832/ifor1189-007
Published: Dec 09, 2014 - Copyright © 2014 SISEF

Technical Reports

Neem (Azadirachta indica A. Juss) is a multipurpose Indian tree important to local economy. Conservation of the genetic resources of neem is essential for the adaptability of this tree species to projected climate change impacts. Here, the effect of type and concentration of auxins in different rooting media on adventitious root formation (ARF) in mini-cuttings of Azadirachta indica is depicted. Three different rooting media (i.e., sand, vermiculite and soil) were used, and the experiment was established using three types of auxin (IBA, IAA and NAA) and 6 concentration treatment combinations (100, 250, 500, 750, 1000 and 1500 mg l-1), in a complete randomized block design (CRBD). Significant effects of different auxin types, concentration treatments and rooting media on adventitious root formation of neem mini-cuttings were observed. Mini-cuttings were assessed for rooting percentage, number of roots, root length and number of leaves. IBA resulted in higher rooting percentage (90%), number of roots (149.56), root length (14.83 cm) and number of leaves per rooted mini-cuttings (12.78), when growing in sand. The determination of proper rooting protocols and the use of mini-cuttings were proved important for improving mass propagation of A. indica.


Mini-cuttings, Adventitious Root Formation (ARF), Azadirachta Indica, Auxin

Authors’ address

Ashok Gehlot
Rajesh Kumar Gupta
Inder Dev Arya
Sarita Arya
Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur 342001, Rajasthan (India)
Atul Tripathi
Regional Pesticides Testing Laboratory, Department of Agriculture and Co-operation, Chandigarh (India)

Corresponding author

Ashok Gehlot


Gehlot A, Gupta RK, Arya ID, Arya S, Tripathi A (2014). De novo adventitious root formations in mini-cuttings of Azadirachta indica in response to different rooting media and auxin treatments. iForest 8: 558-564. - doi: 10.3832/ifor1189-007

Academic Editor

Gianfranco Minotta

Paper history

Received: Nov 28, 2013
Accepted: Nov 13, 2014

First online: Dec 09, 2014
Publication Date: Aug 02, 2015
Publication Time: 0.87 months

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