iForest - Biogeosciences and Forestry


Use of overburden waste for London plane (Platanus × acerifolia) growth: the role of plant growth promoting microbial consortia

Vera Karličić (1), Danka Radić (1)   , Jelena Jovičić-Petrović (1), Blažo Lalević (1), Filis Morina (2), Vesna Golubović Curguz (3), Vera Raičević (1)

iForest - Biogeosciences and Forestry, Volume 10, Issue 4, Pages 692-699 (2017)
doi: https://doi.org/10.3832/ifor2135-010
Published: Jul 17, 2017 - Copyright © 2017 SISEF

Research Articles

Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus × acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara’s overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.


Plant Growth Promoting Bacteria, London Plane, Overburden Waste, Revegetation

Authors’ address

Vera Karličić
Danka Radić
Jelena Jovičić-Petrović
Blažo Lalević
Vera Raičević
Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun (Serbia)
Filis Morina
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, Belgrade (Serbia)
Vesna Golubović Curguz
Faculty of Forestry, University of Belgrade, Kneza Višeslava 1, Belgrade (Serbia)

Corresponding author



Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Morina F, Curguz VGć, Raičević V (2017). Use of overburden waste for London plane (Platanus × acerifolia) growth: the role of plant growth promoting microbial consortia. iForest 10: 692-699. - doi: 10.3832/ifor2135-010

Academic Editor

Claudia Cocozza

Paper history

Received: Jun 14, 2016
Accepted: Apr 12, 2017

First online: Jul 17, 2017
Publication Date: Aug 31, 2017
Publication Time: 3.20 months

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