iForest - Biogeosciences and Forestry


Conservation of Betula oycoviensis, an endangered rare taxon, using vegetative propagation methods

Jan Vítámvás (1)   , Ivan Kuneš (1), Iva Viehmannová (2), Rostislav Linda (1), Martin Baláš (1)

iForest - Biogeosciences and Forestry, Volume 13, Issue 2, Pages 107-113 (2020)
doi: https://doi.org/10.3832/ifor3243-013
Published: Mar 23, 2020 - Copyright © 2020 SISEF

Research Articles

Ojcow birch (Betula oycoviensis Besser) is a rare Central European tree taxon, micro-populations of which are found in only several localities. With a view to maintaining the B. oycoviensis gene pool, this study tested the species’ potential for micropropagation, grafting, and propagation by cuttings. Plant material for vegetative propagation was collected from ten genotypes in the Czech Republic. In vitro culture was established from axillary buds surfaces sterilized with 0.1% HgCl2 and cultivated on woody plant (WP) medium supplemented with 1 mg l-1 6-benzylaminopurine (BAP). Two genotypes of the species were successfully multiplied by in vitro propagation using WP medium supplemented with 0-2 mg l-1 BAP. The BAP concentration of 1 mg l-1 proved to be optimal, yielding 2.5 new shoots per explant in genotype 516 and 3.5 shoots per explant in genotype 545. The shoots were rooted on half-strength Murashige and Skoog (MS) medium supplemented with various concentrations of α-naphthylacetic acid (NAA) and indole-3-butyric acid (IBA). The highest rooting percentages (72.5% and 77.5% for genotypes 516 and 545, respectively) were achieved on the medium with the combination of both auxins at concentrations of 0.3 mg l-1. The rooted plants were transferred ex vitro in substrate composed of sand, peat, and perlite (1:1:1) and acclimated in the greenhouse. After 4 weeks, more than 90% of plants survived. Grafting was carried out in spring using Betula pendula as rootstock. The efficiency of this technique ranged from 0% to 50% across genotypes, and 4 out of 10 genotypes were successfully propagated by grafting. The cuttings were treated with commercial root stimulators Stimulax I and Stimulator AS-1, planted in a mixture of peat and sand (1:1) in the greenhouse, and watered regularly. This technique resulted in 0% rooting, however, and no cutting survived until the end of the vegetation period. The results of this study show that protocols for in vitro propagation and grafting can be employed for effective mass propagation of B. oycoviensis, although these processes show genotype-dependent responses.


Betula, Cutting, Grafting, In vitro Propagation, Rooting

Authors’ address

Jan Vítámvás 0000-0001-8181-8151
Ivan Kuneš 0000-0002-1875-384X
Rostislav Linda 0000-0002-9602-7915
Martin Baláš
Czech University of Life Sciences Prague, Faculty of Forestry and Wood Sciences, Kamýcká 1176, Praha 6 Suchdol (Czech Republic)
Iva Viehmannová 0000-0002-2540-7443
Czech University of Life Sciences, Faculty of Tropical AgriSciences, Kamýcká 129, Praha 6 Suchdol (Czech Republic)

Corresponding author

Jan Vítámvás


Vítámvás J, Kuneš I, Viehmannová I, Linda R, Baláš M (2020). Conservation of Betula oycoviensis, an endangered rare taxon, using vegetative propagation methods. iForest 13: 107-113. - doi: 10.3832/ifor3243-013

Academic Editor

Werther Guidi Nissim

Paper history

Received: Sep 18, 2019
Accepted: Jan 21, 2020

First online: Mar 23, 2020
Publication Date: Apr 30, 2020
Publication Time: 2.07 months

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