iForest - Biogeosciences and Forestry


Stomata morphological traits in two different genotypes of Populus nigra L.

Giuseppe Russo (1)   , Paolo De Angelis (2), James E Mickle (3), Maria Rosaria Lumaga Barone (4)

iForest - Biogeosciences and Forestry, Volume 8, Issue 4, Pages 547-551 (2014)
doi: https://doi.org/10.3832/ifor1104-007
Published: Sep 16, 2014 - Copyright © 2014 SISEF

Short Communications

Populus nigra L. (black poplar) possesses amphistomatic leaves, with large (giant) and normal sized stomata. The role of giant stomata in leaf development, and the consequences on stomatal density in adult leaves remains elusive. This paper describes the characteristics of ordinary and giant stomata in leaves of two black poplar genotypes (58-861 with large leaves from northern Italy, and Poli with small leaves from southern Italy). Stomatal traits in both genotypes were studied using light microscopy on mature leaf adaxial and abaxial epidermal impressions. Moreover, scanning electron microscopy was applied to study giant and normal stomata in early, young, and mature leaves. Leaf abaxial surfaces in the two genotypes revealed variable sizes and patterns of stomata related to differences in intrinsic water use efficiency (Wi). These observations provided evidence of different stomatal types in mature black poplar leaves, and new information regarding the presence and potential role of giant stomata in black poplar leaves.


Giant Stomata, Poplar Clones, Genotypes, Populus, SEM

Authors’ address

Giuseppe Russo
Istituto di Biologia Agroambientale e Forestale (IBAF), Consiglio Nazionale delle Ricerche (CNR), Porano, TR (Italy)
Paolo De Angelis
Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Università della Tuscia, Viterbo (Italy)
James E Mickle
Department of Plant and Microbial Biology, North Carolina State University, Box 7612, 27692-7612 Raleigh, NC (USA)
Maria Rosaria Lumaga Barone
Dipartimento di Biologia, Orto Botanico, Università degli Studi Napoli “Federico II”, Naples (Italy)

Corresponding author



Russo G, De Angelis P, Mickle JE, Lumaga Barone MR (2014). Stomata morphological traits in two different genotypes of Populus nigra L.. iForest 8: 547-551. - doi: 10.3832/ifor1104-007

Academic Editor

Roberto Tognetti

Paper history

Received: Aug 13, 2013
Accepted: May 23, 2014

First online: Sep 16, 2014
Publication Date: Aug 02, 2015
Publication Time: 3.87 months

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