There is an increasing interest in using plant growth-promoting rhizobacteria (PGPR) to improve hybrid poplar performance under water stress conditions. We assessed the growth and leaf-level physiological responses of different hybrid poplar clones to the inoculation of a microbial consortium and subjected to moderate water shortage. In a nursery experiment, growth, leaf gas exchange, and biomass partitioning traits were assessed during one growing season on twenty hybrid clones from interspecific crosses of Populus trichocarpa × P.deltoides and (P. trichocarpa × P. deltoides) × P. deltoides, which were submitted to two treatments of PGPM inoculation (Inoculated, Inoc_1 vs. non-inoculated, Inoc_0) and two irrigation treatments (full water vs. water restriction). The water restriction decreased shoot growth, photosynthetic rate (Asat), and stomatal conductance (gs); increased intrinsic water use efficiency (WUEint) of the hybrid clones but it did not change the root-to-shoot ratio. Unlike our expectations, by the end of the study, treatment Inoc_1 slightly decreased basal diameter (D) and height (H) relative to Inoc_0 (5.8% and 5.2 %, respectively). Moreover, seven clones significantly decreased the root biomass by 37% to 62% in the Inoc_1 relative to Inoc_0 treatment, while the other clones showed no response to the inoculation. Oppositely, while most of the clones showed no response to the Inoc_1 treatment on leaf-physiological traits compared to Inoc_0, some of them exhibited an increase of Asat of 15% to 39%. Overall, the consortium applied did not improve the responses to the water restriction, and responses to the inoculation were more associated with a deleterious than a growth-promoting effect, which is discussed in the context of nutrient immobilization, application method, and timing.
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Yáñez MA, Espinoza S, Ovalle J, Magni C, Martínez-Herrera E (2023). Variations in the performance of hybrid poplars subjected to the inoculation of a microbial consortium and water restriction. iForest 16: 352-360. - doi: 10.3832/ifor4378-016
Academic Editor
Claudia Cocozza
Paper history
Received: May 11, 2023
Accepted: Sep 30, 2023
First online: Dec 13, 2023
Publication Date: Dec 31, 2023
Publication Time: 2.47 months
© SISEF - The Italian Society of Silviculture and Forest Ecology 2023
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