Influence of inorganic salts on biomass production, biochemical composition, and bioethanol production of Populus alba

doi:10.3832/ifor3438-013

Influence of inorganic salts on biomass production, biochemical composition, and bioethanol production of Populus alba

Seon Jeong Sim⁽¹⁾, Seong Hyeon Yong⁽²⁾, DongJin Park⁽³⁾, Eunji Choi⁽²⁾, Yuwon Seol⁽²⁾, Hyun Jin Song⁽³⁾, Mi Jin Jeong⁽⁴⁾, Hak Gon Kim⁽¹⁾, Myung Suk Choi^(2-5)

iForest - Biogeosciences and Forestry, Volume 13, Issue 6, Pages 566-574 (2020)
doi: https://doi.org/10.3832/ifor3438-013
Published: Dec 07, 2020 - Copyright © 2020 SISEF

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Inorganic salts are very important for the biosynthesis of major components such as cellulose and lignin. In order to investigate biomass production, major components of the biosynthesis of plant cell wall and the bioethanol production of Populus alba, we examined the effect of inorganic salts on in vitro culture systems without specific mineral salts. The medium without H₂PO₄^- was supportive for Populus alba shoot growth, while the absence of NH₄⁺ resulted in poor shoot growth. The medium without H₂PO₄^-and Fe³⁺inhibited above-ground biomass production, whereas NH₄⁺and K⁺deprivation led to an enhancement of the same. The root/shoot ratio of Populus alba in the medium without H₂PO₄^- was high compared with plants cultured in the control medium. H₂PO₄^- is deeply involved in lignin biosynthesis, and its removal has been shown to reduce the biosynthesis of lignin. Plants grown on nitrate-free medium were found to be good for enzymatic saccharification and ethanol production. The plants grown in the medium without NO₃^- showed 72.0% enzyme digestibility, and the yield of ethanol showed 9.58% ethanol productivity after 12 hours. These results can be used as the basis for producing high-quality biomass for future bioethanol production.

Keywords

Enzymatic Hydrolysis, Fermentation, Inorganic Salts, In vitro Culture, Populus alba

Authors’ address

(1)

Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute, Jinju, 52615 (South Korea)

(2)

0000-0001-8567-5004
0000-0002-6191-686X
0000-0002-6794-3404
0000-0003-1464-1573
Division of Environmental Forest Science, Gyeongsang National University, Jinju, 52828 (South Korea)

(3)

0000-0002-6089-4064

Department of Seed and Seedling Management, National Forest Seed Variety Center, Chungju 27495 (South Korea)

(4)

Plant Conservation Division, Korea National Arboretum of the Korea Forest Service, Pocheon 11186 (South Korea)

(5)

0000-0003-1464-1573
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam, 52828 (South Korea)

Corresponding author

Myung Suk Choi
bioforest@naver.com

Citation

Sim SJ, Yong SH, Park DJ, Choi E, Seol Y, Song HJ, Jeong MJ, Kim HG, Choi MS (2020). Influence of inorganic salts on biomass production, biochemical composition, and bioethanol production of Populus alba. iForest 13: 566-574. - doi: 10.3832/ifor3438-013

Academic Editor

Federica Brunoni

Paper history

Received: Apr 06, 2020
Accepted: Sep 28, 2020

First online: Dec 07, 2020
Publication Date: Dec 31, 2020
Publication Time: 2.33 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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