The use of branch enclosures to assess direct and indirect effects of elevated CO2 on photosynthesis, respiration and isoprene emission of Populus alba leaves

doi:10.3832/ifor0429-0010049

We used a novel system of branch enclosures to study the impact of elevated CO₂ (900 ppm) on the gas-exchange characteristics of developed and developing leaves of white poplar (Populus alba L.), as well as of leaves subsequently developing at ambient CO₂, outside the enclosures in which the CO₂ concentration was raised. We found no significant effect of elevated CO₂ on photosynthesis, respiration and isoprene emission, as the rates of developed and developing leaves inside the enclosures, and of leaves developing outside the enclosures, were similar to those recorded using enclosures maintained at ambient CO₂. The enclosure system, however, largely influenced the rates of gas-exchange. In fact, leaves already developed inside the enclosures showed rates of photosynthesis, stomatal conductance, and isoprene emission higher than leaves developing inside the enclosures, and also higher than leaves developing outside the enclosure. These differences were caused by a higher efficiency in the light use and by a higher Ribulose 1.5 bisphosphate carboxylase (Rubisco) activity in leaves fully developed inside enclosures than in the other leaf classes. The experiment overall suggests that branch enclosures may alter the physiology of the plants, reducing or counteracting the impact of elevated CO₂, which we predicted to stimulate photosynthesis and uncouple isoprene emission from photosynthesis. This may be an important bias against the use of enclosure systems for studies of the impact of environmental constraints and global change factors on physiological features.

Keywords

Populus alba, Elevated CO₂, Branch Enclosure, Photosynthesis, Isoprene Emission

Authors’ address

(1)

CNR - Istituto di Biologia Agroambientale e Forestale, v. Salaria Km. 29.300, I-00016 Monterotondo Scalo, RM (Italy)

(2)

CNR - Istituto sull’Inquinamento Atmosferico, v. Salaria Km. 29.300, I-00016 Monterotondo Scalo, RM (Italy)

Corresponding author

Federico Brilli
federico.brilli@ibaf.cnr.it

Citation

Brilli F, Tricoli D, Fares S, Centritto M, Loreto F (2008). The use of branch enclosures to assess direct and indirect effects of elevated CO₂ on photosynthesis, respiration and isoprene emission of Populus alba leaves. iForest 1: 49-54. - doi: 10.3832/ifor0429-0010049

Paper history

Received: Mar 09, 2006
Accepted: Jan 01, 2007

First online: Feb 28, 2008
Publication Date: Feb 28, 2008
Publication Time: 14.10 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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