Chloroplast microsatellites as a tool for phylogeographic studies: the case of white oaks in Poland

doi:10.3832/ifor1597-008

Chloroplast microsatellites as a tool for phylogeographic studies: the case of white oaks in Poland

Michal Chmielewski⁽¹⁾, Katarzyna Meyza⁽¹⁾, Igor J Chybicki⁽¹⁾, Artur Dzialuk⁽¹⁾, Monika Litkowiec⁽²⁾, Jaroslaw Burczyk⁽¹⁾

iForest - Biogeosciences and Forestry, Volume 8, Issue 6, Pages 765-771 (2015)
doi: https://doi.org/10.3832/ifor1597-008
Published: Jul 19, 2015 - Copyright © 2015 SISEF

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Assessing the distribution of chloroplast DNA (cpDNA) haplotype variation is useful for studying the phylogeography of angiosperms. In the last two decades the cpDNA phylogeography of white oaks in Europe has been extensively studied, mostly based on the PCR-RFLP technique. However, PCR-RFLPs have low mutation rates and are primarily useful for reconstructing patterns at large geographical scales and lack resolution at fine spatial scales. Here we evaluate the usefulness of chloroplast microsatellites (cpSSR) as an alternative to PCR-RFLPs in Polish oak populations which have been underrepresented in previous studies. Eighty-five cpSSR haplotypes were detected using 14 cpSSR loci and a broad collection of 6680 trees sampled throughout Poland. Haplotype diversity was significantly lower in Q. petraea (H_e= 0.798) than in Q. robur (H_e = 0.820). Only 17 haplotypes (H01-H17) were found in 13 or more individuals, comprising together 97.9% of the sample. Most frequent cpSSR haplotypes were related to PCR-RFLP haplotypes, establishing the cross-references between the two marker systems. There was significant concordance between the matrices of genetic distances obtained by PCR-RFLP haplotypes and cpSSR haplotypes. Phylogenetic relationships among cpSSR haplotypes supported the existence of the three predominant maternal lineages of oaks in Poland: Iberian (7.8%), Apennine (20.6%) and Balkan (65.5%). The results are discussed with regards to the usefulness of cpSSR markers for phylogeographic studies.

Keywords

Chloroplast Microsatellites, PCR-RFLP, White Oaks, Phylogeography

Authors’ address

(1)

Institute of Experimental Biology, Department of Genetics, Kazimierz Wielki University of Bydgoszcz, Chodkiewicza 30, 85-064 Bydgoszcz (Poland)

(2)

Institute of Dendrology, Polish Academy of Sciences, ul. Parkowa 5, 62-035 Kórnik (Poland)

Corresponding author

Jaroslaw Burczyk
burczyk@ukw.edu.pl

Citation

Chmielewski M, Meyza K, Chybicki IJ, Dzialuk A, Litkowiec M, Burczyk J (2015). Chloroplast microsatellites as a tool for phylogeographic studies: the case of white oaks in Poland. iForest 8: 765-771. - doi: 10.3832/ifor1597-008

Academic Editor

Andrea Piotti

Paper history

Received: Feb 12, 2015
Accepted: Jun 01, 2015

First online: Jul 19, 2015
Publication Date: Dec 01, 2015
Publication Time: 1.60 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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