The largest and most economically important conifer genus Pinus is widespread in the northern hemisphere. Comprehensive phylogenies relying on complete chloroplast gene sequences are now available for the entire genus. However, phylogenetic relationships remain unresolved for certain lineages. One such example, which is also inconsistent in terms of biogeography, is within the subsection Pinus and includes five taxa: Pinus densiflora, P. nigra, P. resinosa, P. sylvestris and P. mugo / uncinata species complex. In this study, we use this clade as an example to explain weak support in phylogenetic studies of closely related pine species and show that some of the most popular genetic markers, namely the chloroplast DNA barcoding sequences matK, psbA- trnH and rbcL, are not recommended for species identification purposes in European pines. In addition, we show that matK and psbA-trnH contain contradicting phylogenetic signals in some of the most economically important pine species.
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Citation
Olsson S, Giovannelli G, Roig A, Spanu I, Vendramin GG, Fady B (2022). Chloroplast DNA barcoding genes matK and psbA-trnH are not suitable for species identification and phylogenetic analyses in closely related pines. iForest 15: 141-147. - doi: 10.3832/ifor3913-015
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Alberto Santini
Paper history
Received: Jun 25, 2021
Accepted: Feb 15, 2022
First online: Apr 25, 2022
Publication Date: Apr 30, 2022
Publication Time: 2.30 months
© SISEF - The Italian Society of Silviculture and Forest Ecology 2022
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