Effects of abiotic stress on gene transcription in European beech: ozone affects ethylene biosynthesis in saplings of Fagus sylvatica L.

doi:10.3832/ifor0495-002

Effects of abiotic stress on gene transcription in European beech: ozone affects ethylene biosynthesis in saplings of Fagus sylvatica L.

GA Betz^(1-2), E Gerstner⁽¹⁾, M Olbrich⁽¹⁾, JB Winkler⁽³⁾, C Langebartels⁽¹⁾, W Heller⁽¹⁾, H Sandermann^(1-4), D Ernst⁽¹⁾

iForest - Biogeosciences and Forestry, Volume 2, Issue 3, Pages 114-118 (2009)
doi: https://doi.org/10.3832/ifor0495-002
Published: Jun 10, 2009 - Copyright © 2009 SISEF

Research Articles

Collection/Special Issue: COST Action E52 Meeting 2008 - Florence (Italy)
Evaluation of beech genetic resources for sustainable forestry
Guest Editors: Georg von Wühlisch, Raffaello Giannini

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The influence of ozone (150-190 nl L^-1; 8h/d) on transcription levels of genes involved in the biosynthesis of the stress hormone ethylene, and its precursor 1-aminocyclopropane-1-carboxylate (ACC), was analysed in leaves of European beech saplings. Ozone-induced leaf lesions appeared 7 weeks after onset of ozone exposure. Cell lesion formation was preceded by persistent increases in ethylene emission, in the level of its malonylated precursor ACC, and in the transcript levels of specific ACC synthase 1 (ACS1), ACS2, ACC oxidase 1 (ACO1), and ACO2. Our results demonstrate that mechanisms similar to those operating in herbaceous plants may determine beech saplings responses to ozone exposure.

Keywords

Abiotic stress, Ethylene biosynthesis, Fagus sylvatica, Gene expression, Ozone

Authors’ address

(1)

Institute of Biochemical Plant Pathology “Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg (Germany)

(2)

Olympus Life Science Research Europa GmbH, Sauerbruchstraße 50, D-81377 München (Germany)

(3)

Department of Environmental Engineering, Helmholtz Zentrum München - German Research Center for Environmental Health, D-85764 Neuherberg (Germany)

(4)

Ecotox Freiburg, Schubertstr. 1, D-79104 Freiburg (Germany)

Corresponding author

D Ernst
ernst@helmholtz-muenchen.de

Citation

Betz GA, Gerstner E, Olbrich M, Winkler JB, Langebartels C, Heller W, Sandermann H, Ernst D (2009). Effects of abiotic stress on gene transcription in European beech: ozone affects ethylene biosynthesis in saplings of Fagus sylvatica L.. iForest 2: 114-118. - doi: 10.3832/ifor0495-002

Academic Editor

Marco Borghetti

Paper history

Received: Feb 04, 2009
Accepted: Mar 12, 2009

First online: Jun 10, 2009
Publication Date: Jun 10, 2009
Publication Time: 3.00 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Betz G, Knappe C, Lapierre C, Olbrich M, Welzl G, Langebartels C, Heller W, Sandermann H, Ernst D (2009a)
Ozone affects shikimate pathway transcripts and monomeric lignin composition in European beech (Fagus sylvatica L.). European Journal of Forest Research 128: 109-116.
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(2)

Betz AG, Gerstner E, Stich S, Winkler B, Welzl G, Kremmer E, Langebartels C, Heller W, Sandermann H, Ernst D (2009b)
Ozone affects shikimate pathway genes and secondary metabolites of European beech (Fagus sylvatica L.) grown under greenhouse conditions. Trees online first.
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