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Changes in the proteome of juvenile European beech following three years exposure to free-air elevated ozone

R Kerner (1-2)   , JB Winkler (4), JW Dupuy (3), M Jürgensen (2), C Lindermayr (2), D Ernst (2), G Müller-Starck (1)

iForest - Biogeosciences and Forestry, Volume 4, Issue 2, Pages 69-76 (2011)
doi: https://doi.org/10.3832/ifor0570-004
Published: Apr 05, 2011 - Copyright © 2011 SISEF

Research Articles

Collection/Special Issue: IUFRO RG 7.01 2010 - Antalya (Turkey)
Adaptation of Forest Ecosystems to Air Pollution and Climate Change
Guest Editors: Elena Paoletti, Yusuf Serengil


Tropospheric ozone, one of the most phytotoxic air pollutants, may specially impose in long-lived forest trees substantial reduction in productivity and biomass. European beech saplings grown in lysimeter around areas were used to monitor proteomic changes upon elevated ozone concentrations following four vegetation periods of exposure. A proteome study based on highly sensitive two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was performed to identify protein changes in European beech, the most important deciduous tree in Central Europe. Main emphasis was on identifying differentially expressed proteins after long-time period of ozone exposure under natural conditions rather than short-term responses or reactions under controlled conditions. Our results clearly demonstrate a response of European beech saplings to long-term ozone fumigation at the protein level. We indicate changes in the protein abundance of 142 protein spots; among them 59 were increased and 83 decreased following three years of elevated ozone exposure. As the first step, 40 proteins were identified by a homology driven mass spectrometric approach. Some of the identified proteins have been previously described in the context of short-term ozone responses in plants, indicating, at least for certain cellular functions, the congruence of plant reactions following short- and long-term ozone exposure. Under elevated ozone exposure, abundance of proteins related to the Calvin cycle and photosynthetic electron transport chain were decreased whereas the abundance of proteins regarding the carbon metabolism/catabolism were increased.

  Keywords


Abiotic stress, Elevated ozone, European beech, Woody plants, 2-D DIGE

Authors’ address

(1)
R Kerner
G Müller-Starck
Section of Forest Genetics, Technische Universität München, Hans-Carl-von-Carlowitz-Platz 2, D-85354 Freising (Germany)
(2)
R Kerner
M Jürgensen
C Lindermayr
D Ernst
Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg (Germany)
(3)
JW Dupuy
Université de Bordeaux, Centre de Génomique Fonctionnelle Bordeaux, Plateforme Protéome, F-33000 Bordeaux (France)
(4)
JB Winkler
Department of Environmental Engineering, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg (Germany).

Corresponding author

 

Citation

Kerner R, Winkler JB, Dupuy JW, Jürgensen M, Lindermayr C, Ernst D, Müller-Starck G (2011). Changes in the proteome of juvenile European beech following three years exposure to free-air elevated ozone. iForest 4: 69-76. - doi: 10.3832/ifor0570-004

Paper history

Received: Sep 13, 2010
Accepted: Jan 17, 2011

First online: Apr 05, 2011
Publication Date: Apr 05, 2011
Publication Time: 2.60 months

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