iForest - Biogeosciences and Forestry


Tracing the acclimation of European beech (Fagus sylvatica L.) populations to climatic stress by analyzing the antioxidant system

Eszter Visi-Rajczi (1)   , Tamás Hofmann (1), Levente Albert (1), Csaba Mátyás (2)

iForest - Biogeosciences and Forestry, Volume 14, Issue 2, Pages 95-103 (2021)
doi: https://doi.org/10.3832/ifor3542-013
Published: Mar 01, 2021 - Copyright © 2021 SISEF

Research Articles

Through a common garden (provenance) experiment, we investigated the metabolic responses to climatic stress with regard to the acclimation potential of different European beech (Fagus sylvatica L.) populations. Selected enzymatic and non-enzymatic antioxidants were analyzed in leaves. Peroxidase (POD) and polyphenol oxidase (PPO) enzyme activity, total protein content as well as ABTS [2.2’-azino-bis-(3-etylbenzothiazoline)-6-sulphonic acid] antioxidant capacity were measured in the leaves of selected populations. Major leaf polyphenols were identified and their relative amounts were compared. Significant correlations were found between phenotypic (diameter growth) response to simulated climatic stress and the activity (and amount) of selected chemical components. The concentrations of certain polyphenols, POD enzyme activity, and total protein content may be chemical indicators of the acclimation potential of populations and may contribute to the forecasting of climate change effects, which can aid in the selection of suitable propagation material for adaptive silviculture.


Beech, Drought Stress, Antioxidants, Phenotypic Plasticity, Provenance Trial, Climate Change

Authors’ address

Eszter Visi-Rajczi 0000-0003-0723-9885
Tamás Hofmann 0000-0002-1928-7879
Levente Albert
Institute of Chemistry, University of Sopron, Sopron, 9400 (Hungary)
Csaba Mátyás 0000-0001-6095-7611
Institute of Environmental and Earth Sciences, University of Sopron, Sopron, 9400 (Hungary)

Corresponding author



Visi-Rajczi E, Hofmann T, Albert L, Mátyás C (2021). Tracing the acclimation of European beech (Fagus sylvatica L.) populations to climatic stress by analyzing the antioxidant system. iForest 14: 95-103. - doi: 10.3832/ifor3542-013

Academic Editor

Claudia Cocozza

Paper history

Received: Jun 04, 2020
Accepted: Dec 24, 2020

First online: Mar 01, 2021
Publication Date: Apr 30, 2021
Publication Time: 2.23 months

Breakdown by View Type

(Waiting for server response...)

Article Usage

Total Article Views: 25742
(from publication date up to now)

Breakdown by View Type
HTML Page Views: 22296
Abstract Page Views: 1401
PDF Downloads: 1684
Citation/Reference Downloads: 2
XML Downloads: 359

Web Metrics
Days since publication: 1174
Overall contacts: 25742
Avg. contacts per week: 153.49

Article Citations

Article citations are based on data periodically collected from the Clarivate Web of Science web site
(last update: Feb 2023)

Total number of cites (since 2021): 6
Average cites per year: 2.00


Publication Metrics

by Dimensions ©

Articles citing this article

List of the papers citing this article based on CrossRef Cited-by.

Achmadi SS (2019)
Polyphenols resources in Indonesia from economic perspective. In: “Polyphenols in Plants (2nd edn). Isolation, Purification and Extract Preparation” (Watson RR ed). Academic press, San Diego, CA, USA, pp. 67-79.
CrossRef | Gscholar
Allen CD, Breshears DD, McDowell NG (2015)
On underestimation of global vulnerability to tree mortality and forest die-off from hotter drought in the Anthropocene. Ecosphere 6: 1-55.
CrossRef | Gscholar
Aranda I, Sánchez-Gómez D, De Miguel M, Mancha JA, Guevara MA, Cadahía E, Fernández De Simón MB (2017)
Fagus sylvatica L. provenances maintain different leaf metabolic profiles and functional response. Acta Oecologica 82: 1-9.
CrossRef | Gscholar
Bae JJ, Choo YS, Ono K, Sumida A, Hara T (2010)
Photoprotective mechanisms in cold-acclimated and nonacclimated needles of Picea glehnii. Photosynthetica 48: 110-116.
CrossRef | Gscholar
Baniulis D, Sirgediene M, Haimi P, Tamošiune I, Danusevičius D (2020)
Constitutive and cold acclimation-regulated protein expression profiles of scots pine seedlings reveal potential for adaptive capacity of geographically distant populations. Forests 11: 89.
CrossRef | Gscholar
Berini JL, Brockman SA, Hegeman AD, Reich PB, Muthukrishnan R, Montgomery RA, Forester JD (2018)
Combinations of abiotic factors differentially alter production of plant secondary metabolites in five woody plant species in the boreal-temperate transition zone. Frontiers in Plant Science 9: 1257.
CrossRef | Gscholar
Berki I, Rasztovits E, Móricz N, Mátyás C (2009)
Determination of the drought tolerance limit of beech forests and forecasting their future distribution in Hungary. Cereal Research Communications 37: 613-616.
CrossRef | Gscholar
Boughalleb F, Mhamdi M (2011)
Possible involvement of proline and the antioxidant defense systems in the drought tolerance of three olive cultivars grown under increasing water deficit regimes. Agricultural Journal 6: 378-391.
CrossRef | Gscholar
Bradford MM (1976)
A rapid sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
CrossRef | Gscholar
Cadahía E, Fernández De Simón B, Aranda I, Sanz M, Sánchez-Gómez D, Pint E (2015)
Non-targeted metabolomic profile of Fagus sylvatica L. leaves using liquid chromatography with mass spectrometry and gas chromatography with mass spectrometry. Phytochemical Analysis 26: 171-182.
CrossRef | Gscholar
Caudullo G, Welk E, San-Miguel-Ayanz J (2017)
Chorological maps for the main European woody species. Data in Brief 12: 662-666.
CrossRef | Gscholar
Chakhchar A, Lamaoui M, Wahbi S, Ferradous A, El Mousadik A, Ibnsouda-Koraichi S, Filali-Maltouf A, El Modafar C (2015a)
Differential drought tolerance of four contrasting Argania spinosa ecotypes assessed by enzymatic and non-enzymatic antioxidant. International Journal of Recent Scientific Research 6: 3002-3009.
Chakhchar A, Wahbi S, Lamaoui M, Ferradous A, El Mousadik A, Ibnsouda-Koraichi S, Filali-Maltouf A, El Modafar C (2015b)
Physiological and biochemical traits of drought tolerance in Argania spinosa. Journal of Plant Interactions 10: (1) 252-261.
CrossRef | Gscholar
Comps B, Thiébault B, Merzeau D, Letouzey J (1990)
Allozymic variability in beechwoods (Fagus sylvatica L.) over Central Europe: spatial differentiation among and within stands. Heredity 65: 407-417.
CrossRef | Gscholar
Comps B, Mátyás Cs Geburek J, Letouzey J (1998)
Genetic variation in beech populations along the Alps chain and in the Hungarian Basin. Forest Genetics 5: 1-9.
Online | Gscholar
Czúcz B, Gálhidy L, Mátyás C (2011)
Present and forecasted xeric climatic limits of beech and sessile oak distribution at low altitudes in Central Europe. Annals of Forest Science 68 (1): 99-108.
CrossRef | Gscholar
Dalmagro AP, Camargo A, Filho HHS, Valcanaia MM, De Jesus PC, Zeni ALB (2018)
Seasonal variation in the antioxidant phytocompounds production from the Morus nigra leaves. Industrial Crops and Products 123: 323-330.
CrossRef | Gscholar
Del Río LA (2015)
ROS and RNS in plant physiology: an overview. Journal of Experimental Botany 66 (10): 2827-37.
CrossRef | Gscholar
Di Ferdinando M, Brunetti C, Fini A, Tattini M (2012)
Chapter 9 - Flavonoids as antioxidants in plants under abiotic stresses. In: “Abiotic Stress Responses in Plants” (Ahmad P, Prasad MNV eds). Springer, New York, USA, pp. 159-179.
CrossRef | Gscholar
Dixon RA, Paiva NL (1995)
Stress-induced phenylpropanoid metabolism. The Plant Cell 7: 1085-1097.
CrossRef | Gscholar
Ellenberg H (1988)
Vegetation ecology of Central Europe (4th edn). Cambridge University Press, Cambridge, UK, pp. 731.
CrossRef | Gscholar
Feucht W, Treutter D (1999)
The role of flavan-3-ols and proanthocyanidins in plant defense. In: “Principles and Practices of Plant Ecology” (Inderjit, Dakshini KMM, Foy CL eds). CRC Press LLC, Boca Raton, FL, USA, pp. 308-332.
Feucht W, Treutter D, Christ E (1994)
Accumulation of flavanols in yellowing beech leaves from forest decline sites. Tree Physiology 14: 403-412.
CrossRef | Gscholar
Feucht W, Treutter D, Christ E (1996)
Flavanols in grapevine: in vitro accumulation and defence reactions in shoots. Vitis 35: 113-118.
Feucht W, Treutter D, Christ E (1997)
Role of flavanols in yellowing trees of the Black Forest. Tree Physiology 17: 335-340.
CrossRef | Gscholar
Flurkey WH, Jen JJ (1978)
Peroxidase and polyphenol oxidase activities in developing peaches. Journal of Food Science 43: 1826-1829.
CrossRef | Gscholar
Foyer C, Noctor G (2005)
Oxidant and antioxidant signaling in plants: a reevaluation of the concept of oxidative stress in a physiological context. Plant, Cell and Environment 28 (8): 1056-1071.
CrossRef | Gscholar
Grace SC, Logan BA (1996)
Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiology 112: 1631-1640.
CrossRef | Gscholar
Gupta DK, Palma JM, Corpas FJ (2018)
Antioxidants and antioxidant enzymes in higher plants. Springer International Publishing, eBook, 300.
CrossRef | Gscholar
Haberer K, Herbinger K, Alexou M, Rennenberg H, Tausz M (2008)
Effects of drought and canopy ozone exposure on antioxidants in fine roots of mature European beech (Fagus sylvatica). Tree Physiology 28: 713-719.
CrossRef | Gscholar
Hijmans RJ, Cameron SE, Parra JL, Jones PG, Jarvis A (2005)
Very high resolution interpolated climate surfaces for global land areas. International Journal of Climatology 25: 1965-1978.
CrossRef | Gscholar
Hofmann T, Albert L, Rétfalvi T, Visi-Rajczi E, Brolly G (2008)
TLC analysis of the in vitro reaction of beech (Fagus sylvatica L.) wood enzyme extract with catechins. Journal of Planar Chromatography 21: 83-88.
CrossRef | Gscholar
Hofmann T, Nebehaj E, Albert L (2015)
The high-performance liquid chromatography/multistage electrospray mass spectrometric investigation and extraction optimization of beech (Fagus sylvatica L.) bark polyphenols. Journal of Chromatography A 1393: 96-105.
CrossRef | Gscholar
Hofmann T, Tálos-Nebehaj E, Albert L (2017a)
Leaf polyphenols as indicators of climatic adaptation of beech (Fagus sylvatica L.) - an HPLC-MS/MS via MRM approach. International Labmate 42 (3): 12-14.
Online | Gscholar
Hofmann T, Tálos-Nebehaj E, Albert L, Németh L (2017b)
Antioxidant efficiency of beech (Fagus sylvatica L.) bark polyphenols assessed by chemometric methods. Industrial Crops and Products 108: 26-35.
CrossRef | Gscholar
Horváth A, Mátyás C (2014)
Növedékcsökkenés elörevetítése egy bükk származási kísérlet alapján [Estimation of increment decline caused by climate change, based on data of a beech provenance trial]. Erdészettudományi Közlemények 4: 91-99. [in Hungarian with English summary]
Horváth A, Mátyás C (2016)
The decline of vitality caused by increasing drought in a beech provenance trial predicted by juvenile growth. South-east European Forestry 7 (1): 21-28.
CrossRef | Gscholar
Isah T (2019)
Stress and defense responses in plant secondary metabolites production. Biological Research 52: 39.
CrossRef | Gscholar
Kala S, Godara AK (2011)
Effect of moisture stress on leaf proteins, proline and free amino acid content in commercial cultivars of Ziziphus mauritiana. Journal of Scientific Research 55: 65-69.
Korotaeva N, Romanenko A, Suvorova G, Ivanova MV, Lomovatskaya L, Borovskii G, Voinikov V (2015)
Seasonal changes in the content of dehydrins in mesophyll cells of common pine needles. Photosynthesis Research 124: 159-169.
CrossRef | Gscholar
Lakatos F, Molnár M (2009)
Mass mortality of beech (Fagus sylvatica L.) in south-west Hungary. Acta Silvatica et Lignaria Hungarica 5: 75-82.
Online | Gscholar
Laukkanen H, Haggman H, Kontunen-Soppela S, Hohtola A (1999)
Tissue browning of in vitro cultures of Scots pine: role of peroxidase and polyphenol oxidase. Physiologia Plantarum 106: 337-343.
CrossRef | Gscholar
Lee OH, Lee BY, Lee J, Lee H, Son J, Park C, Shetty K, Kim YC (2009)
Assessment of phenolics enriched extract and fractions of olive leaves and their antioxidant activities. Bioresource Technology 100: 6107-6113.
CrossRef | Gscholar
Li S, Zhou L, Addo-Danso SD, Ding G, Sun M, Wu S, Lin S (2020)
Nitrogen supply enhances the physiological resistance of Chinese fir plantlets under polyethylene glycol (PEG)-induced drought stress. Scientific Reports 10: 7509.
CrossRef | Gscholar
Linnakoski R, Kasanen R, Dounavi A, Forbes KM (2019)
Editorial: forest health under climate change: effects on tree resilience, and pest and pathogen dynamics. Frontiers in Plant Science 10: 1157.
CrossRef | Gscholar
Luwe M (1996)
Antioxidants in the apoplast and symplast of beech (Fagus sylvatica L.) leaves: seasonal variations and responses to changing ozone concentrations in air. Plant Cell and Environment 19: 321-328.
CrossRef | Gscholar
Mátyás C (1994)
Modelling climate change effects with provenance test data. Tree Physiology 14: 797-804.
CrossRef | Gscholar
Mátyás C, Berki I, Czúcz B, Gálos B, Móricz N, Rasztovits E (2010)
Future of beech in Southeast Europe from the perspective of evolutionary ecology. Acta Silvatica et Lignaria Hungarica 6: 91-110.
Online | Gscholar
Münzbergová Z, Latzel V, Surinová M, Hadincová V (2018)
DNA methylation as a possible mechanism affecting ability of natural populations to adapt to changing climate. Oikos 128: 124-134.
CrossRef | Gscholar
Pedan V, Fischer N, Rohn S (2016)
An online NP-HPLC-DPPH method for the determination of the antioxidant activity of condensed polyphenols in cocoa. Food Research International 89 (2): 890-900.
CrossRef | Gscholar
Pennycooke JC, Cox S, Stushnoff C (2005)
Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia × hybrida). Environmental and Experimental Botany 53 (2): 225-232.
CrossRef | Gscholar
Penuelas J, Ogaya R, Boada M, Jump AS (2007)
Migration, invasion and decline: changes in recruitment and forest structure in a warming-linked shift of European beech forest in Catalonia (NE Spain). Ecography 30: 829-837.
CrossRef | Gscholar
Popovic BM, Stajner D, Zdero-Pavlovic R, Tumbas-Saponjac V, Canadanovic-Brunet J, Orlovic S (2016)
Water stress induces changes in polyphenol profile and antioxidant capacity in poplar plants (Populus spp.). Plant Physiology and Biochemistry 105: 242-250.
CrossRef | Gscholar
Pukacki PM, Kaminska-Rozek E (2013)
Reactive species, antioxidants and cold tolerance during deacclimation of Picea abies populations. Acta Physiologiae Plantarum 35: 129-138.
CrossRef | Gscholar
Sáenz-Romero C, Kremer A, Nagy L, Ujvári-Jármay E, Ducousso A, Kóczán-Horváth A, Hansen JK, Mátyás C (2019)
Common garden comparisons confirm inherited differences in sensitivity to climate change between forest tree species. PeerJ 7: e6213.
CrossRef | Gscholar
Selye H (1950)
Stress and the general adaptation syndrome. British Medical Journal 4667: 1383-1392.
CrossRef | Gscholar
Shannon LM, Kay E, Lew JY (1966)
Peroxidase isoenzymes from horseradish roots. Journal of Biological Chemistry 241 (9): 2166-2172.
CrossRef | Gscholar
Sies H (1991)
Oxidative stress: from basic research to clinical application. The American Journal of Medicine 91 (3C): 31-38.
CrossRef | Gscholar
Smirnoff N (2005)
Antioxidants and reactive oxygen species in plants. Wiley-Blackwell Publishing Ltd, Oxford, UK, pp. 320.
CrossRef | Gscholar
Song YY, Simard SW, Caroll A, Mohn WW, Zheng R (2015)
Defoliation of interior Douglas fir elicits carbon transfer and defense signaling to ponderosa pine neighbors through ectomycorrhizal networks. Scientific Reports 5 (8495): 1-9.
CrossRef | Gscholar
Stajner D, Orlovic S, Popovic BM, Kebert M, Stojnic S, Klasnja B (2013)
Chemical parameters of oxidative stress adaptability in Beech. Journal of Chemistry 2013: 1-8.
CrossRef | Gscholar
Stojanovic DB, Kric A, Matovic B, Orlovic S, Duputie A, Djurdjevic V, Galiç Z, Stojnic S (2013)
Prediction of the European beech (Fagus sylvatica L.) xeric limit using a regional climate model: an example from southeast Europe. Agricultural and Forest Meteorology 176: 94-103.
CrossRef | Gscholar
Stratil P, Klejdus B, Kuban V (2007)
Determination of phenolic compounds and their antioxidant activity in fruits and cereals. Talanta 71: 1741-1751.
CrossRef | Gscholar
Tanase C, Cosarca S, Muntean D-L (2019)
A critical review of phenolic compounds extracted from the bark of woody vascular plants and their potential biological activity. Molecules 24 (6): 1182.
CrossRef | Gscholar
Tang W, Newton RJ (2004)
Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginia pine (Pinus virginiana Mill.). Plant Science 167: 621-628.
CrossRef | Gscholar
Vahdati K, Lotfi N (2013)
Abiotic stress tolerance in plants with emphasizing on drought and salinity stresses in walnut. In: “Abiotic Stress-plant Responses and Applications in Agriculture” (Vahdati K, Leslie C eds). InTech, Rijeka, Croatia, pp. 307-367.
CrossRef | Gscholar
Visiné Rajczi E, Hofmann T, Albert L, Mátyás C (2018)
Az antioxidáns rendszer, mint a bükk (Fagus sylvatica L.) klimatikus alkalmazkodóképességének lehetséges indikátora [Antioxidant system as a potential indicator of the climatic adaptation of beech (Fagus sylvatica L) Erdészettudományi Közlemények 8 (2): 25-35. [in Hungarian with English summary and captions]
CrossRef | Gscholar
Von Wuehlisch G, Alia R (2011)
COST E52 final report - Genetic resources of European beech (Fagus sylvatica L.) for sustainable forestry. In: Proceedings of the “COST E52 - Evaluation of beech genetic resources for sustainable forestry”. Monografías INIA, Serie Forestal 22, pp. 148.
Zhang S, Chen L, Duan B, Korpelainen H, Li C (2012)
Populus cathayana males exhibit more efficient protective mechanisms than females under drought stress. Forest Ecology and Management 275: 68-78.
CrossRef | Gscholar
Zhang S, Li L, Cui Y, Luo L, Li Y, Zhou P, Sun B (2017)
Preparative high-speed counter-current chromatography separation of grape seed proanthocyanidins according to degree of polymerization. Food Chemistry 219: 399-407.
CrossRef | Gscholar
Zheng Y, Yang Q, Xu M, Chi Y, Shen R, Li P, Dai H (2012)
Responses of Pinus massoniana and Pinus taeda to freezing in temperate forests in central China. Scandinavian Journal of Forest Research 27: 520-531.
CrossRef | Gscholar
Zolfaghari R, Hosseini SM, Korori SAA (2010)
Relationship between peroxidase and catalase with metabolism and environmental factors in Beech (Fagus orientalis Lipsky) in three different elevations. International Journal of Environmental Sciences 1: 243-252.

This website uses cookies to ensure you get the best experience on our website. More info