A new approach to ozone plant fumigation: the Web-O3-Fumigation. Isoprene response to a gradient of ozone stress in leaves of Quercus pubescens

doi:10.3832/ifor0240-0010022

The innovative Web-O₃-Fumigation (WOF) is a suitable technique to study O₃ effects in plants. Through WOF, a very high dose of O₃ fumigation may be obtained at the level of selected leaves, while allowing a gradient of ozone stress in branches at different distance from branch directly fumigated with O₃ diffusing from perforated Teflon tubes. We used WOF to study the impact of O₃ on the emission of isoprene, a putatively powerful antioxidant, in leaves of Quercus pubescens. In our experiment we produced with WOF: i) approximately 300 ppb O₃ on the leaves of the branch directly exposed to the pollutant; ii) approximately 190 ppb on the leaves of the branches distant less than 30 cm from the treated branch; iii) a summer ambient O₃ concentration (100 ppb) on the leaves of the branches distant more than 30 cm from the branch directly fumigated. High O₃ concentrations induced a long-lasting inhibition of photosynthesis and isoprene emission in leaves which were directly fumigated. However, isoprene emission was stimulated by intermediate O₃ concentration 288 hours after the end of the treatment. Isoprene stimulation, and the activation of the related antioxidant mechanism, may therefore be dependent on the O₃ concentration and may be different in plant canopies depending on their exposure to the pollutant.

Keywords

Ozone, Isoprene, Web-fumigation, Photosynthesis, Stomatal conductance

Authors’ address

(1)

Istituto di Biologia Agroambientale e Forestale - CNR, v. Salaria Km. 29.300, 00016 Monterotondo Scalo, RM (Italy)

Corresponding author

P Pinelli
paola.pinelli@unimol.it

Citation

Pinelli P, Tricoli D (2008). A new approach to ozone plant fumigation: the Web-O₃-Fumigation. Isoprene response to a gradient of ozone stress in leaves of Quercus pubescens. iForest 1: 22-26. - doi: 10.3832/ifor0240-0010022

Paper history

Received: Sep 10, 2004
Accepted: Dec 20, 2004

First online: Feb 28, 2008
Publication Date: Feb 28, 2008
Publication Time: 38.83 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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