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iForest - Biogeosciences and Forestry

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Chitosan exhibits variable effects on pine (Pinus sylvestris L.) and alder (Alnus glutinosa L.) growth and secondary metabolism

Milana Šilanskiene (1), Vaida Sirgedaite-Šežiene (1), Doroteja Vaitiekunaite (1)   , MKDS Co. (2)

iForest - Biogeosciences and Forestry, Volume 17, Issue 4, Pages 236-244 (2024)
doi: https://doi.org/10.3832/ifor4404-017
Published: Aug 06, 2024 - Copyright © 2024 SISEF

Research Articles


Chitosan is a naturally abundant biopolymer that has been studied as an alternative means to elicit beneficial responses in plants, such as enhanced plant growth and improved defense response. Most studies focus on agriculture and horticulture, but few have investigated forest trees. Based on chitosan’s success as a biostimulant, we hypothesized that trees such as pine (Pinus sylvestris L.) and alder (Alnus glutinosa L.) may also be positively affected by it. We evaluated vegetative growth parameters and secondary metabolites (phenolics, antioxidant indicators), pigments of seedlings grown in sterile in vitro and non-sterile ex vitro conditions to determine if various concentrations of chitosan solubilized in different solvents and stored in different conditions would significantly impact trees at various stages and conditions of growth. Results showed that in in vitro conditions, chitosan had a mainly positive effect on pine germination and growth, but a mainly negative effect on pine secondary metabolism (SM) indicators. In vitro grown alder’s SMs were positively affected in all measured parameters, but chitosan had no effect on growth. Overall, ex vitro results indicated that chitosan has no effect on pine growth and varied impact on its SM indicators. Similarly, the effect on growth parameters and SM indicators was varied in alder. Results on how chitosan storage can affect its impact on tree growth showed that storage conditions had a significant impact on both pine and alder. Based on our data, future studies must carefully evaluate all possible variables to optimize chitosan use in silviculture.

  Keywords


Forest, Tree, Chitosan, Growth Improvement, Secondary Metabolites

Authors’ address

(1)
Milana Šilanskiene
Vaida Sirgedaite-Šežiene 0000-0002-1607-0551
Doroteja Vaitiekunaite 0000-0003-2775-9802
Lithuanian Research Centre for Agriculture and Forestry, Institute of Forestry, Liepu St. 1, Girionys, LT-53101 Kaunas dist. (Lithuania)
(2)
MKDS Co.
MKDS Innovation Co., Piliakalnio st. 68, Nemenčine, LT-15175 Vilniaus distr. (Lithuania)

Corresponding author

 
Doroteja Vaitiekunaite
doroteja.vaitiekunaite@lammc.lt

Citation

Šilanskiene M, Sirgedaite-Šežiene V, Vaitiekunaite D, Co. MKDS (2024). Chitosan exhibits variable effects on pine (Pinus sylvestris L.) and alder (Alnus glutinosa L.) growth and secondary metabolism. iForest 17: 236-244. - doi: 10.3832/ifor4404-017

Academic Editor

Claudia Cocozza

Paper history

Received: Jun 12, 2023
Accepted: Mar 31, 2024

First online: Aug 06, 2024
Publication Date: Aug 31, 2024
Publication Time: 4.27 months

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