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iForest - Biogeosciences and Forestry

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Secondary metabolites of six Siberian and Crimean Armillaria species and their in vitro phytotoxicity to pine, larch and poplar

Tatyana V Antipova (1)   , Valentina P Zhelifonova (1), Yulia A Litovka (2-3), Igor N Pavlov (2-3), Boris P Baskunov (1), Zhanna A Kokh (2-4), Polina V Makolova (2-3), Anton A Timofeev (2), Anatoly G Kozlovsky (1)

iForest - Biogeosciences and Forestry, Volume 15, Issue 1, Pages 38-46 (2022)
doi: https://doi.org/10.3832/ifor3840-014
Published: Feb 04, 2022 - Copyright © 2022 SISEF

Research Articles


Basidiomycetes Armillaria infect deciduous, coniferous and fruit trees, causing enormous economic damage. The role of secondary metabolites (tricyclic sesquiterpene aryl esters - melleolides) in the life cycle and pathogenesis of Armillaria is under active investigation. To date, not all species of Armillaria have been tested for the biosynthesis of melleolides. We investigated the secondary metabolite profiles of six root-pathogenic species of the genus Armillaria (A. borealis Marxmüller & Korhonen, A. cepistipes Velenovský, A. gallica Marxm, A. mellea (Vahl) P. Kummer, A. sinapina Bérubé & Dessur, A. ostoyae (Romagn.) Herink) distributed in Siberia (South Krasnoyarsk Krai, Republic of Tyva, Republic of Khakassia, Taimyr Peninsula), Russian Far East (Sikhote-Alin) and Crimea (Krymsky National Park, Chatyr-Dag Mountain Lower Plateau). A total of 15 compounds were identified in the metabolome profile. Two compounds (melleolide D and melledonal C) are synthesized by all investigated strains irrespective of their geographic location and host plant. The maximum spectrum of melleolides (7-8 compounds) was found in isolates of A. borealis, A. gallica, A. sinapina, A. ostoyae. In submerged culture, the maximum accumulation of melleolides varied from 2 up to 239 mg l-1. A mixture of melleolide D and melledonal C (1:1) synthesized by the most productive strain A. mellea Cr2-17 was first found to have a phytotoxic action on the growth parameters of the callus culture Populus balsamifera and 10-day-old conifer seedlings. A 0.5% concentration of melleolides caused a credible decrease of P. balsamifera callus raw biomass; a decrease of the viability of Larix sibirica and, which is especially significant, Pinus sylvestris seedlings; inhibition of stem and root growth processes; dechromation of foliage; loss of turgor. The occurrence of a broad range of melleolides in the metabolome profile and two common compounds in all investigated strains, with a phytotoxic action at their sufficiently high concentration, enables considering the synthesis of melleolides by Armillaria fungi as one of the possible mechanisms of their pathogenicity efficiently realized in strains characterized by overproduction of melleolides under natural conditions.

  Keywords


Melleolides, Metabolome, Armillaria fungi, Phytotoxicity, Callus, Coniferous Plants

Authors’ address

(1)
Tatyana V Antipova 0000-0002-4860-2647
Valentina P Zhelifonova 0000-0001-9213-9584
Boris P Baskunov 0000-0002-0342-2431
Anatoly G Kozlovsky
GK Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC Pushchino Centre for Biological Research, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, 142290 (Russia)
(2)
Yulia A Litovka 0000-0001-5343-7896
Igor N Pavlov 0000-0001-7312-0933
Zhanna A Kokh 0000-0003-4016-7596
Polina V Makolova
Anton A Timofeev
VN Sukachev Institute of Forest, FRC KSC, Siberian Branch, Russian Academy of Sciences, 50 Akademgorodok Str., Bld 28, Krasnoyarsk, 660036 (Russia)
(3)
Yulia A Litovka 0000-0001-5343-7896
Igor N Pavlov 0000-0001-7312-0933
Polina V Makolova
FSBEIHE MF Reshetnev Siberian State University of Science and Technology, 82 Prosp. Mira, Krasnoyarsk, 660037 (Russia)
(4)
Zhanna A Kokh 0000-0003-4016-7596
FSBEIHE Krasnoyarsk State Agrarian University, 90 Prosp. Mira, Krasnoyarsk, 660049 (Russia)

Corresponding author

 
Tatyana V Antipova
tatantip@rambler.ru

Citation

Antipova TV, Zhelifonova VP, Litovka YA, Pavlov IN, Baskunov BP, Kokh ZA, Makolova PV, Timofeev AA, Kozlovsky AG (2022). Secondary metabolites of six Siberian and Crimean Armillaria species and their in vitro phytotoxicity to pine, larch and poplar. iForest 15: 38-46. - doi: 10.3832/ifor3840-014

Academic Editor

Alberto Santini

Paper history

Received: Apr 06, 2021
Accepted: Dec 02, 2021

First online: Feb 04, 2022
Publication Date: Feb 28, 2022
Publication Time: 2.13 months

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