iForest - Biogeosciences and Forestry


Ectomycorrhizal diversity in a mature pedunculate oak stand near Morović, Serbia

Marina Milović, Branislav Kovačević, Milan Drekić, Andrej Pilipović, Saša Pekeč, Lazar Kesić, Milutin Dilas, Velisav Karaklić, Zoran Galić

iForest - Biogeosciences and Forestry, Volume 16, Issue 6, Pages 345-351 (2023)
doi: https://doi.org/10.3832/ifor4362-016
Published: Nov 22, 2023 - Copyright © 2023 SISEF

Research Articles

Pedunculate oak is among the most economically important deciduous forest tree species in Europe and is also a host for many important ectomycorrhizal (ECM) fungi. The aim of this study was to analyse the ECM fungal community in a mature pedunculate oak stand near Morović, Serbia in spring and autumn. ECM fungi were determined by combining morpho-anatomical characterization of ectomycorrhizas with molecular analysis based on PCR amplification of the internal transcribed spacer region of fungal nuclear ribosomal DNA. The number of ECM fungal taxa and the number of different categories of fine roots were counted, diversity indices were calculated, and ECM fungi were classified into exploration types. Twenty-one ECM fungal taxa were recorded in the studied mature pedunculate oak stand, 19 in spring and 13 in autumn. ECM communities consisted of one dominant taxon and a larger number of rare taxa. Lactarius quietus was the most abundant ECM fungus in both seasons which made association with more than half of ECM root tips. At the stand near Morović, contact exploration type (ET) dominated, short-distance ET was less abundant, while medium-distance fringe ET and long-distance ET were rare in both seasons. The most pronounced difference between seasons is recorded in the number of ECM fungal taxa. The number of ECM fungal taxa and diversity indices recorded in the studied pedunculate oak stand were lower or similar compared to values obtained in stands of oak species across Europe.


Ectomycorrhizal Fungi, Quercus robur L., Morpho-Anatomical Characterization, ITS Region


Milović M, Kovačević B, Drekić M, Pilipović A, Pekeč S, Kesić L, Dilas M, Karaklić V, Galić Z (2023). Ectomycorrhizal diversity in a mature pedunculate oak stand near Morović, Serbia. iForest 16: 345-351. - doi: 10.3832/ifor4362-016

Academic Editor

Daniela Baldantoni

Paper history

Received: Apr 11, 2023
Accepted: Sep 20, 2023

First online: Nov 22, 2023
Publication Date: Dec 31, 2023
Publication Time: 2.10 months

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Agerer R (1991)
Characterisation of ectomycorrhiza. Techniques for the study of mycorrhiza In: “Methods in Microbiology vol. 23” (Norris JR, Read DJ, Varma AK eds). Academic Press, London, UK, pp. 25-72.
Agerer R (2001)
Exploration types of ectomycorrhizae. A proposal to classify ECM mycelial systems according to their patterns of differentiation and putative ecological importance. Mycorrhiza 11 (2): 107-114.
CrossRef | Gscholar
Agerer R (2008)
Colour atlas of ectomycorrhizae (13th edn). Einhorn-Verlag, Schwäbisch Gmünd, Germany.
Agerer R, Rambold G (2022)
DEEMY - An information system for characterization and determination of ectomycorrhizae. München, Germany, web site.
Online | Gscholar
Anthony MA, Crowther TW, Van Der Linde S, Suz LM, Bidartondo MI, Cox F, Schaub M, Rautio P, Ferretti M, Vesterdal L, De Vos B, Dettwiler M, Eickenscheidt N, Schmitz A, Meesenburg H, Andreae H, Jacob F, Dietrich HP, Waldner P, Gessler A, Frey B, Schramm O, Van Den Bulk P, Hensen A, Averill C (2022)
Forest tree growth is linked to mycorrhizal fungal composition and function across Europe. The ISME Journal 16 (5): 1327-1336.
CrossRef | Gscholar
Atlas R, Bartha R (1981)
Introduction to microbiology. Addison-Wesley Publishing Company, Reading, UK, pp. 242-244.
Banković S, Medarević M, Pantić D, Petrović N (2009)
Nacionalna inventura šuma Republike Srbije - šumski fond Republike Srbije [National forest inventory of the Republic of Serbia - Forest growing stock of the Republic of Serbia]. Ministarstvo poljoprivrede šumarstva i vodoprivrede Republike Srbije - Uprava za šume, Beograd, Serbia. [in Serbian]
Barsoum N, O’Hara SW, Cottrell JE, Forster J, San Jose Garcia M, Schonrogge K, Shaw L (2021)
Root ectomycorrhizal status of oak trees symptomatic and asymptomatic for acute oak decline in southern Britain. Forest Ecology Management 482: 118800.
CrossRef | Gscholar
Bartlett MS (1936)
The square root transformation in analysis of variance. Journal of the Royal Statistical Society 3: 68-78.
CrossRef | Gscholar
Bzdyk RM, Olchowik J, Studnicki M, Nowakowska JA, Oszako T, Urban A, Hilszczanska D (2019)
Ectomycorrhizal colonisation in declining oak stands on the Krotoszyn Plateau, Poland. Forests 10 (30): 1-21.
CrossRef | Gscholar
Courty PE, Bréda N, Garbaye J (2007)
Relation between oak tree phenology and the secretion of organic matter degrading enzymes by Lactarius quietus ectomycorrhizas before and during bud break. Soil Biology and Biochemistry 39 (7): 1655-1663.
CrossRef | Gscholar
Courty PE, Franc A, Pierrat JC, Garbaye J (2008)
Temporal changes in the ectomycorrhizal community in two soil horizons of a temperate oak forest. Applied and Environmental Microbiology 74 (18): 5792-5801.
De Román M, De Miguel AM (2005)
Post-fire, seasonal and annual dynamics of the ectomycorrhizal community in a Quercus ilex L. forest over a 3-year period. Mycorrhiza 15: 471-482.
CrossRef | Gscholar
Ducousso A, Bordacs S (2004)
EUFORGEN Technical Guidelines for genetic conservation and use for pedunculate and sessile oaks (Quercus robur and Q. petraea). International Plant Genetic Resources Institute, Rome, Italy, pp. 1-6.
Online | Gscholar
Eaton E, Caudullo G, Oliveira S, De Rigo D (2016)
Quercus robur and Quercus petraea in Europe: distribution, habitat, usage and threats. In: “European Atlas of Forest Tree Species” (San-Miguel-Ayanz J, De Rigo D, Caudullo G, Houston Durrant T, Mauri A eds). Publication Office EU, Luxembourg, pp. 160-163.
Galić Z, Ponjarac R, Kiš A, Novčić Z (2015)
Karakteristike zemljišta u GJ Ristovača [Soil types in management unite Ristovača] Topola/Poplar 195- 196: 5-13. [in Serbian]
Gardes M, Bruns TD (1993)
ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113-118.
CrossRef | Gscholar
Hall IR, Brown GT, Zambonelli A (2007)
Taming the truffle. The history, lore, and science of the ultimate mushroom. Timber Press, Inc., Portland, OR, USA, pp. 304.
Hobbie EA, Agerer R (2010)
Nitrogen isotopes in ectomycorrhizal sporocarps correspond to belowground exploration types. Plant Soil 327: 71-83.
CrossRef | Gscholar
Katanić M, Marković M, Pap P, Zlatković M, Pekeč S, Kovačević B (2017)
Biologija i uzgoj tartufa u svetu i Srbiji [Biology and cultivation of truffles in the world and Serbia]. Topola/Poplar 199/ 200: 175-190. [In Serbian]
Keizer PJ, Arnolds E (1994)
Succession of ectomycorrhizal fungi in roadside verges planted with common oak (Quercus robur L.) in Drenthe, The Netherlands. Mycorrhiza 4: 147-159.
CrossRef | Gscholar
Kesić L, Stojnić S, Orlović S, Pavlović L, Lozjanin R, Tepavac A, Vaštag E (2018)
Varijabilnost morfoloških karaktera žira hrasta lužnjaka (Quercus robur L.), kitnjaka (Q. petraea (Matt.) Lieb.) i cera (Q. cerris L.) [Variability of the morphological charateristics of pedunculate (Quercus robur L.), sessile (Q. petraea (Matt.) Lieb.) and Turkey oak (Q. cerris L.) acorns]. Topola/Poplar 201/ 202: 187-201. [In Serbian]
Kovacs G, Pausch M, Urban A (2000)
Diversity of ectomycorrhizal morphotypes and oak decline. Phyton 40 (4): 109-116.
Kraigher H (1996)
Tipi mikorize: taksonomija, pomen, aplikacija [Types of ectomycorrhizae - their taxonomy, role, and application]. Zbornik Gozdarstva in Lesarstva 49: 33-66. [In Slovenian]
Kraigher H, Petkovšek SA (2011)
Mycobioindication of stress in forest ecosystems. In: “Diversity and Biotechnology of Ectomycorrhizae, vol. 25” (Rai M, Varma A eds), Soil Biology, Springer, Berlin, Heidelberg, Germany, pp. 301-322.
CrossRef | Gscholar
Kyaschenko J, Clemmensen KE, Hagenbo A, Karltun E, Lindahl BD (2017)
Shift in fungal communities and associated enzyme activities along an age gradient of managed Pinus sylvestris stands. The ISME Journal 11 (4): 863-874.
CrossRef | Gscholar
Last FT, Dighton J, Mason PA (1987)
Successions of sheathing mycorrhizal fungi. Trends in Ecology and Evolution 2: 157-161.
Lilleskova EA, Hobbie EA, Horton TR (2011)
Conservation of ectomycorrhizal fungi: exploring the linkages between functional and taxonomic responses to anthropogenic N deposition. Fungal Ecology 4: 174-183.
CrossRef | Gscholar
Milović M, Kebert M, Orlović S (2021a)
How mycorrhizas can help forests to cope with ongoing climate change? Sumarski List 145 (5-6): 279-286.
CrossRef | Gscholar
Milović M, Vasić V, Drekić M, Kovačević B, Pekeč S, Galić Z, Orlović S (2021b)
Diversity of ectomycorrhizal fungi associated with Quercus petraea in the national park Fruška Gora in the Republic of Serbia. Sumarski List 145(11-12): 547-555.
CrossRef | Gscholar
Milović M, Orlović S, Grebenc T, Bajc M, Kovačević B, Kraigher H (2021c)
Ectomycorrhizal fungal community in mature white poplar plantation. iForest 14: 540-547.
CrossRef | Gscholar
Milović M, Kovačević B, Pekeč S, Pilipović A, Kesić L, Gavranović Markić A, Orlović S (2022)
Diversity of ectomycorrhizal fungi in young pedunculate oak stand from Morović, Serbia. South-east European forestry 13 (1): 19-25.
CrossRef | Gscholar
NCBI (2021)
GenBank. National Center for Biotechnology Information - NCBI, Bethesda, ML, USA, web site.
Online | Gscholar
Nilsson RH, Larsson K-H, Taylor AFS, Bengtsson-Palme J, Jeppesen TS, Schigel D, Kennedy P, Picard K, Glöckner FO, Tedersoo L, Saar I, Kõljalg U, Abarenkov K (2018)
The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications. Nucleic Acids Research 47: 259-26.
CrossRef | Gscholar
O’Hanlon R, Harrington TJ (2012)
Similar taxonomic richness but different communities of ectomycorrhizas in native forests and non-native plantation forests. Mycorrhiza 22 (5): 371-382.
CrossRef | Gscholar
Olchowik J, Hilszczanska D, Bzdyk RM, Studnicki M, Malewski T, Borowski Z (2019)
Effect of deadwood on ectomycorrhizal colonisation of old-growth oak forests. Forests 10 (6): 480.
CrossRef | Gscholar
Radević V, Pap P, Vasić V (2020)
Gazdovanje šumama hrasta lužnjaka u Ravnom Sremu: juče, danas, sutra [Management of the common oak forests in Ravni Srem: yesterday, today, tomorrow]. Topola/Poplar 206: 41-52 [In Serbian]
Rosinger C, Sandén H, Matthews B, Mayer M, Godbold DL (2018)
Patterns in ectomycorrhizal diversity, community composition, and exploration types in European beech, pine, and spruce forests. Forests 9 (8): 445.
CrossRef | Gscholar
RSHS (2020)
Home page. Hydrometeorological Service - RSHS, Beograd, Republic of Serbia, web site.
Online | Gscholar
Schirkonyer U, Bauer C, Rothe G (2013)
Ectomycorrhizal diversity at five different tree species in forests of the Taunus Mountains in Central Germany. Open Journal of Ecology 3 (1): 66-81.
CrossRef | Gscholar
Selosse MA, Richard F, He X, Simard SW (2006)
Mycorrhizal networks: les liaisons dangereuses? Trends in Ecology and Evolution 21 (11): 621-628.
CrossRef | Gscholar
Smith SE, Read DJ (2008)
Mycorrhizal symbiosis (3rd edn). ElsevierAcademic Press, London, UK, pp. 787.
Snedecor W, Cochran WG (1976)
Statistical methods. Iowa State University Press, IA, USA, pp. 524.
Stojanović DB, Orlović S, Zlatković M, Kostić S, Vasić V, Miletić B, Kesić L, Matović B, Boanić D, Pavlović L, Milović M, Pekeč S (2021)
Climate change within Serbian forests: current state and future perspectives. Topola/Poplar 208: 39-56.
Suz LM, Barsoum N, Benham S, Dietrich HP, Fetzer KD, Fischer R, García P, Gehrman J, Kristöfel F, Manninger M, Neagu S, Nicolas M, Oldenburger J, Raspe S, Sánchez G, Schröck HW, Schubert A, Verheyen K, Verstraeten A, Bidartondo MI (2014)
Environmental drivers of ectomycorrhizal communities in Europe’s temperate oak forests. Molecular Ecology 23 (22): 5628-5644.
CrossRef | Gscholar
Taylor AFS, Martin F, Read DJ (2000)
Fungal diversity in ectomycorrhizal communities of Norway spruce (Picea abies (L.) Karst.) and beech (Fagus sylvatica L.) along north-south transects in Europe. In: “Carbon and Nitrogen Cycling in European Forest Ecosystems” (Schulze ED ed). Ecological Studies 142, Springer-Verlag, Berlin Heidelberg, Germany, pp. 343-365.
Voríšková J, Brabcová V, Cajthaml T, Baldrian P (2014)
Seasonal dynamics of fungal communities in a temperate oak forest soil. New Phytologist 201 (1): 269-278.
CrossRef | Gscholar
White TJ, Bruns T, Lee S, Taylor J (1990)
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: “PCR Protocols. A Guide to Methods and Applications” (Innis MA, Gelfand DH, Sninsky JJ, White TJ eds). Academic Press, San Diego, CA, USA, pp. 315-322.
CrossRef | Gscholar

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