Identification of the ambrosia beetle Anisandrus dispar (Fabricius) (Coleoptera Curculionidae Scolytinae) using TaqMan™ probe assay on biological samples

doi:10.3832/ifor4287-016

Identification of the ambrosia beetle Anisandrus dispar (Fabricius) (Coleoptera Curculionidae Scolytinae) using TaqMan™ probe assay on biological samples

Domenico Rizzo⁽¹⁾, Andrea D’Agostino⁽²⁾, Igor Stabile⁽²⁾, Chiara Ranaldi⁽²⁾, Andrea Marrucci⁽²⁾, Claudia Gabriela Zubieta⁽²⁾, Daniele Da Lio⁽²⁾, Linda Bartolini⁽¹⁾, Fabrizio Pennacchio⁽³⁾, Elisabetta Rossi⁽²⁾, Antonio P Garonna⁽⁴⁾

iForest - Biogeosciences and Forestry, Volume 16, Issue 3, Pages 182-187 (2023)
doi: https://doi.org/10.3832/ifor4287-016
Published: Jun 30, 2023 - Copyright © 2023 SISEF

Research Articles

PDF Version

Full Text

Citation

The European shot-hole borer Anisandrus dispar (Fabricius) (Coleoptera Curculionidae Scolytinae) is a well-known ambrosia beetle living on shrubs and several ornamental and fruiting trees where it can cause heavy damages. Like other harmful xyleborine species, A. dispar can represent a potential threat outside its native region. Molecular diagnostic tools can lead to accurate identification of xylophagous species hidden in wooden matrix in phytosanitary surveys at entry points. A molecular assay based on qPCR TaqMan™ Probes was developed for the identification of A. dispar from different matrices. To setup and perform the test, DNA extraction was carried out from adults, larvae, and artificial samples of wood chips from oak healthy plants whose lysates were contaminated with a known amount of DNA of A. dispar adults. The assay has proven inclusive for A. dispar, and exclusive towards the non-target organisms, showing 100% analytical specificity. The limit of detection was 0.32 pg µL^-1 for the samples of insect adult DNA of A. dispar, and at 0.8 pg µL^-1 for the samples containing lysates of Quercus spp. and 0.1 ng µL^-1 of A. dispar adult DNA. Repeatability and reproducibility showed low values independently from the matrix used for DNA extraction, confirming the possible use in diagnostics of biological samples even if not directly related to the presence of A. dispar developmental stages. The presented approach may be adjusted and applied for phytosanitary purposes to other quarantine pests and rapidly detect possible infestations in vegetal matrices globally traded.

Keywords

European Shot-hole Borer, Xyleborini, Quarantine Species, Molecular Diagnostics, Biological Traces

Authors’ address

(1)

0000-0003-2834-4684

Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, v. Ciliegiole 99, I-51100 Pistoia (Italy)

(2)

0000-0002-9222-9501
0000-0002-6073-1046
Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, v. del Borghetto 80, I-56124 Pisa (Italy)

(3)

0000-0001-6609-4386
CREA - Research Centre for Plant Protection and Certification, v. Lanciola 12/A, I-50125 Florence (Italy)

(4)

0000-0002-8441-5208
Department of Agricultural Sciences, University of Naples “Federico II”, v. Università 100, I-80055 Portici (Italy)

(5)

retired.

Corresponding author

Antonio P Garonna
garonna@unina.it

Citation

Rizzo D, D’Agostino A, Stabile I, Ranaldi C, Marrucci A, Zubieta CG, Da Lio D, Bartolini L, Pennacchio F, Rossi E, Garonna AP (2023). Identification of the ambrosia beetle Anisandrus dispar (Fabricius) (Coleoptera Curculionidae Scolytinae) using TaqMan™ probe assay on biological samples. iForest 16: 182-187. - doi: 10.3832/ifor4287-016

Academic Editor

Matteo Marchioro

Paper history

Received: Dec 09, 2022
Accepted: Mar 28, 2023

First online: Jun 30, 2023
Publication Date: Jun 30, 2023
Publication Time: 3.13 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Breakdown by View Type

(Waiting for server response...)

Article Usage

Total Article Views: 9053
(from publication date up to now)

Breakdown by View Type
HTML Page Views: 7682
Abstract Page Views: 688
PDF Downloads: 606
Citation/Reference Downloads: 3
XML Downloads: 74

Web Metrics
Days since publication: 303
Overall contacts: 9053
Avg. contacts per week: 209.15

Article Citations

Article citations are based on data periodically collected from the Clarivate Web of Science web site
(last update: Feb 2023)

(No citations were found up to date. Please come back later)

(no Plumx Metrics available for this paper)

Publication Metrics

by Dimensions ^©

Articles citing this article

List of the papers citing this article based on CrossRef Cited-by.

(1)

Albo JE, Marelli JP, Puig AS (2020)
Rapid molecular identification of Scolytinae (Coleoptera: Curculionidae). International Journal of Molecular Science 20 (23): 5944.
CrossRef | Gscholar

(2)

Amini S, Nozari J, Hosseini R, Rahati R (2020)
Comparison of different DNA extraction methods for the molecular study of bark beetles (Coleoptera: Curculionidae, Scolytinae). Journal of Insect Biodiversity and Systematics 6 (1): 113-124.
CrossRef | Gscholar

(3)

Avtzis DN, Lakatos F (2021)
Bark and wood boring insects. past, present, and the future know ledge we need. Insects 12: 28.
CrossRef | Gscholar

(4)

Briski E, Ghabooli S, Bailey SA, Maclsaac HJ (2016)
Are genetic databases sufficiently populated to detect non-indigenous species? Biological Invasions 18: 1911-1922.
CrossRef | Gscholar

(5)

Bucini D, Balestra GM, Pucci C, Paparatti B (2005)
Bio-ethology of Anisandrus dispar F. and its possible involvement in dieback (Moria) diseases of hazelnut (Corylus avellana L.) plants in Central Italy. Acta Horticulturae 686: 435-444.
CrossRef | Gscholar

(6)

Cognato AI, Sari G, Smith SM, Beaver RA, Li Y, Hulcr J, Jordal BH, Kajimura H, Lin CS, Pham TH, Singh S, Sittichaya W (2020)
The essential role of taxonomic expertise in the creation of DNA databases for the identification and delimitation of Southeast Asian ambrosia beetle species (Curculionidae: Scolytinae: Xyleborini). Frontiers in Ecology and Evolution 8: 27.
CrossRef | Gscholar

(7)

Colombari F, Martinez Sañudo I, Battisti A (2022)
First report of the alien ambrosia beetle Cnestus mutilatus and further finding of Anisandrus maiche in the EPPO region (Coleoptera: Curculionidae: Scolytinae: Xyleborini). EPPO Bulletin 00: 1-5.
CrossRef | Gscholar

(8)

EPPO (2021)
PM 7/98 (5) Specific requirements for laboratories preparing accreditation for a plant pest diagnostic activity. Bulletin OEPP/EPPO 51: 468-498.
CrossRef | Gscholar

(9)

EPPO (2022)
First record of the ambrosia beetle Anisandrus maiche in Switzerland. EPPO Reporting Service no. 07, pp. 148.
Online | Gscholar

(10)

French JRJ, Roeper RA (1975)
Studies on the biology of the ambrosia beetle Xyleborus dispar (F.) (Coleoptera: Scolytidae). Zeitschrift für Angewandte Entomologie 78: 241-247.
CrossRef | Gscholar

(11)

Galko J, Nikolov C, Kimoto T, Kunca A, Gubka A, Vakula J, Zúbrik M, Ostrihon M (2014)
Attraction of ambrosia beetles to ethanol baited traps in a Slovakian oak forest. Biologia 69: 1376-1383.
CrossRef | Gscholar

(12)

Gohli J, Selvarajah T, Kirkendall LR, Jordal BL (2016)
Globally distributed Xyleborus species reveal recurrent intercontinental dispersal in a landscape of ancient worldwide distributions. BMC Evolutionary Biology 16: 37.
CrossRef | Gscholar

(13)

Gomez DF, Rabaglia RJ, Fairbanks KEO, Hulcr J (2018)
North American Xyleborini north of Mexico: a review and key to genera and species (Coleoptera, Curculionidae, Scolytinae). ZooKeys 768: 19-68.
CrossRef | Gscholar

(14)

Ide T, Kanzaki N, Ohmura W, Okabe K (2016a)
Molecular identification of the western drywood termite (Isoptera: Kalotermitidae) by loop-mediated isothermal amplification of DNA from fecal pellets. Journal of Economic Entomology 109 (3): 2234-2237.
CrossRef | Gscholar

(15)

Ide T, Kanzaki N, Ohmura W, Okabe K (2016b)
Molecular identification of an invasive wood-boring insect Lyctus brunneus (Coleoptera: Bostrichidae: Lyctinae) using frass by loop-mediated isothermal amplification and nested PCR assays. Journal of Economic Entomology 109 (3): 1410-1414.
CrossRef | Gscholar

(16)

Ioos R, Fourrier C, Iancu G, Gordon TR (2009)
Sensitive detection of Fusarium circinatum in pine seed by combining an enrichment procedure with a real-time polymerase chain reaction using dual-labeled probe chemistry. Phytopathology 99: 582-590.
CrossRef | Gscholar

(17)

Katoh K, Standley DM (2013)
MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772-780.
CrossRef | Gscholar

(18)

Li R, Mocka R, Huang Q, Abad J, Hartung J, Kinarda G (2008)
A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods 154: 48-55.
CrossRef | Gscholar

(19)

Mandelshtam MY, Yakushkin EA, Petrov AV (2018)
Oriental ambrosia beetles (Coleoptera: Curculionidae: Scolytinae): new inhabitants of Primorsky krai in Russia. Russian Journal of Biological Invasions 9: 355-365.
CrossRef | Gscholar

(20)

Meurisse N, Rassati D, Hurley BP, Brockerhoff EG, Haack RA (2018)
Common pathways by which non-native forest insects move internationally and domestically. Journal of Pest Science 92: 13-27.
CrossRef | Gscholar

(21)

Pennacchio F, Sabbatini PG, Jucker C, Allegro G, Roversi PF (2012)
A key for the identification of larvae of Anoplophora chinensis, Anoplophora glabripennis and Psacothea hilaris (Coleoptera Cerambycidae Lamiinae) in Europe. REDIA - Journal of Zoology 95: 57-65.
Gscholar

(22)

Poland TM, Rassati D (2019)
Improved biosecurity surveillance of non-native forest insects: a review of current methods. Journal of Pest Science 92: 37-49.
CrossRef | Gscholar

(23)

Rabaglia RJ, Dole SA, Cognato AI (2006)
Review of American Xyleborina (Coleoptera: Curculionidae: Scolyinae) occurring North of Mexico, with an illustrated key. Annals of the Entomological Society of America 99 (6): 1034-1056.
CrossRef | Gscholar

(24)

Rabaglia RJ, Cognato AI, Hoebeke ER, Johnson CW, Labonte JR, Carter ME, Vlack JJ (2019)
Early detection and rapid response: a 10-year summary of the USDA Forest Service program of surveillance for non-native bark and Ambrosia beetles. American Entomologist 65 (1): 29-42.
CrossRef | Gscholar

(25)

Rassati D, Faccoli M, Petrucco Toffolo E, Battisti A, Marini L (2015)
Improving the early detection of alien wood-boring beetles in ports and surrounding forests. Journal of Applied Ecology 52: 50-58.
CrossRef | Gscholar

(26)

Rizzo D, Taddei F, Da Lio D, Nugnes F, Barra E, Stefani L, Bartolini L, Griffo RV, Spigno P, Cozzolino L, Rossi E, Garonna AP (2020a)
Identification of the red-necked longhorn beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on frass. Sustainability 12: 6041.
CrossRef | Gscholar

(27)

Rizzo D, Taddei A, Da Lio D, Bruscoli T, Cappellini G, Bartolini L, Salemi C, Luchi N, Pennacchio F, Rossi E (2020b)
Molecular identification of Anoplophora glabripennis (Coleoptera: Cerambycidae) from frass by loop-mediated isothermal amplification. Journal of Economic Entomology 113 (6): 2911-2919.
CrossRef | Gscholar

(28)

Rizzo D, Da Lio D, Bartolini L, Cappellini G, Bruscoli T, Bracalini M, Benigno A, Salemi C, Del Nista D, Aronadio A, Panzavolta T, Moricca S (2020c)
A duplex real-time PCR with probe for simultaneous detection of Geosmithia morbida and its vector Pityophthorus juglandis. PLoS One 15 (10): e0241109.
CrossRef | Gscholar

(29)

Rizzo D, Da Lio D, Bartolini L, Salemi C, Del Nista D, Aronadio A, Pennacchio F, Binazzi F, Francardi V, Garonna AP, Rossi E (2021)
TaqMan probe assays on different biological samples for the identification of three ambrosia beetle species, Xylosandrus compactus (Eichoff), X. crassiusculus (Motschulsky) and X. germanus (Blandford) (Coleoptera Curculionidae Scolytinae). 3 Biotech 11: 259.
CrossRef | Gscholar

(30)

Ruzzier E, Bani L, Cavaletto G, Faccoli M, Rassati D (2022)
Anisandrus maiche Kurentzov (Curculionidae: Scolytinae), an Asian species recently introduced and now widely established in Northern Italy. BioInvasions Records 11 (3): 652-658.
CrossRef | Gscholar

(31)

Saruhan H, Akyol I (2012)
Monitoring population density and fluctuations of Anisandrus dispar and Xyleborinus saxesenii (Coleoptera: Scolytinae, Curculionidae) in hazelnut orchards. African Journal of Biotechnology 11: 4202-4207.
CrossRef | Gscholar

(32)

Sauvard D (2004)
General biology of bark beetles. Chapter 7. In: “Bark and Wood Boring Insects in Living Trees in Europe: A Synthesis” (Lieutier F, Day K, Battisti A, Gregorie J-C, Evans H eds). Springer, Dordrecht, Netherlands, pp. 63-88.
CrossRef | Gscholar

(33)

Smith SM, Beaver RA, Cognato AI (2020)
A monograph of the Xyleborini (Coleoptera, Curculionidae, Scolytinae) of the Indochinese Peninsula (except Malaysia) and China. Zookeys 983: 1-442.
CrossRef | Gscholar

(34)

Speranza S, Bucini D, Paparatti B (2009)
New observation on biology of European Shot-Hole Borer (Xyleborus dispar (F.)) on hazel in Northern Latium (Central Italy). Acta Horticolturae 845: 539-542.
CrossRef | Gscholar

(35)

Strangi A, Sabbatini Peverieri G, Roversi PF (2013)
Managing outbreaks of the citrus long-horned beetle Anoplophora chinensis (Forster) in Europe: molecular diagnosis of plant infestation. Pest Management Science 69: 627-634.
CrossRef | Gscholar

(36)

Taddei A, Becker M, Berger B, Da Lio D, Feltgen S, König S, Hoppe B, Rizzo D (2021)
Molecular identification of Anoplophora glabripennis (Coleoptera: Cerambycidae) and detection from frass samples based on real-time quantitative PCR. Journal of Plant Diseases and Protection 128: 1587-1601.
CrossRef | Gscholar

(37)

Tanasković S, Marjanović M, Gvozdenac S, Popović N, Drašković G (2016)
Sudden occurrence and harmfulness of Xyleborus dispar (Fabricius) on pear. Contemporary Agriculture 65 (3-4): 57-62.
CrossRef | Gscholar

(38)

Vega FE, Hofstetter RWE (2014)
Bark beetles, biology and ecology of native and invasive species. Academic Press, London, UK, pp. 620.
CrossRef | Gscholar

(39)

Wood SL, Bright DE (1992)
A catalog of Scolytinae and Platypotidae (Coleoptera). Part 2: Taxonomic Index, Volume A. Great Basin Naturalist Memories, pp. 1-833.
Gscholar

(40)

Wu Y, Trepanowski NF, Molongoski JJ, Reagel PF, Lingafelter SW, Nadel H, Myers SW, Ray AM (2017)
Identification of wood-boring beetles (Cerambycidae and Buprestidae) intercepted in trade associated solid wood packaging material using DNA barcoding and morphology. Scientific Reports 7: 40316.
CrossRef | Gscholar

iForest - Biogeosciences and Forestry

Contents

Search

Journal Info

Journal Subjects and Fields

For Authors

For Reviewers

For Readers

SISEF Publishing

Search iForest Contents