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iForest - Biogeosciences and Forestry

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Auxin (IAA) and soluble carbohydrate seasonal dynamics monitored during xylogenesis and phloemogenesis in Scots pine

Marek Fajstavr (1-2)   , Zuzana Paschová (1), Kyriaki Giagli (1), Hanuš Vavrčík (1), Vladimír Gryc (1), Josef Urban (3-4)

iForest - Biogeosciences and Forestry, Volume 11, Issue 5, Pages 553-562 (2018)
doi: https://doi.org/10.3832/ifor2734-011
Published: Sep 01, 2018 - Copyright © 2018 SISEF

Research Articles


The metabolic activity of phytohormones and the accumulation of carbohydrates affect the reactivation of the cambial zone and the radial increment of woody plants. We aimed to monitor the dynamics of indole-3-acetic acid (IAA) concentration and amounts of soluble carbohydrates during xylem and phloem formation of one growing season (2015). Six sample trees of Scots pine (Pinus sylvestris L.), aged 80 years on average, growing in the Sobešice research site (404 m a.s.l.) in the Czech Republic were selected. We obtained microcore samples at weekly intervals by the Trephor tool method for cell formation analysis and spectrophotometric determination of IAA and soluble carbohydrate contents. We found that time of the highest concentration of IAA (last week of April) coincided with time of the maximum number of cells in the cambial zone and highest expansion of the cell enlargement stage. When the IAA concentration was too low to be measured, latewood tracheids started to form, and late phloem sieve cell formation ceased. The highest concentration of soluble carbohydrates was 200.40 ± 21.6 µg GLU per sample (May 14). This coincided with the fastest weekly xylem cell increment. This research shows that IAA and soluble carbohydrate dynamics directly affects xylem and phloem formation.

  Keywords


Pinus sylvestris L., Indole-3-Acetic Acid, Xylem, Phloem, Tracheids, Earlywood, Latewood

Authors’ address

(1)
Marek Fajstavr
Zuzana Paschová
Kyriaki Giagli
Hanuš Vavrčík
Vladimír Gryc
Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 3, 61300 Brno (Czech Republic)
(2)
Marek Fajstavr
The Czech Academy of Sciences, CzechGlobe - Global Change Research Institute, Institute of Plant Water Balance and Biomass Production, Belidla 4a, 60300 Brno (Czech Republic)
(3)
Josef Urban
Department of Forest Botany, Dendrology and Geobiocenology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 3, 61300 Brno (Czech Republic)
(4)
Josef Urban
Siberian Federal University, Svobodnyj Prospect 79, Krasnoyarsk, 660041 Krasnoyarsk (Russia)

Corresponding author

 
Marek Fajstavr
fajstavr.maara@gmail.com

Citation

Fajstavr M, Paschová Z, Giagli K, Vavrčík H, Gryc V, Urban J (2018). Auxin (IAA) and soluble carbohydrate seasonal dynamics monitored during xylogenesis and phloemogenesis in Scots pine. iForest 11: 553-562. - doi: 10.3832/ifor2734-011

Academic Editor

Werther Guidi Nissim

Paper history

Received: Jan 19, 2018
Accepted: Jun 26, 2018

First online: Sep 01, 2018
Publication Date: Oct 31, 2018
Publication Time: 2.23 months

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List of the papers citing this article based on CrossRef Cited-by.

 
(1)
Abramoff MD, Magalhães PJ, Ram SJ (2004)
Image processing with imagej. Biophotonics International 11: 36-42.
Gscholar
(2)
Albalasmeh AA, Berhe AA, Ghezzehei TA (2013)
A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydrate Polymers 97: 253-261.
CrossRef | Gscholar
(3)
Aloni R (1995)
The induction of vascular tissues by auxin and cytokinin. In: “Plant Hormones: Physiology, Biochemistry and Molecular Biology” (Davies PJ ed). Springer, Kluwer, Dordrecht, Netherlands, pp. 531-546.
CrossRef | Gscholar
(4)
Bhalerao RP, Bennett MJ (2003)
The case for morphogens in plants. Nature Cell Biology 5: 939-943.
CrossRef | Gscholar
(5)
Bhalerao RP, Fischer U (2014)
Auxin gradients across wood - instructive or incidental? Physiologia Plantarum 151: 43-51.
CrossRef | Gscholar
(6)
Carlsbecker A, Helariutta Y (2005)
Phloem and xylem specification: pieces of the puzzle emerge. Current Opinion in Plant Biology 8: 512-517.
CrossRef | Gscholar
(7)
Denne MP (1989)
Definition of latewood according to Mork (1928). IAWA Journal 10: 59-62.
CrossRef | Gscholar
(8)
Christensen JH, Hewitson N, Busuioc A, Chen A, Gao X, Held I, Jones R, Kolli RK, Kwon WT, Laprise R, Magana Rueda V, Mearns L, Menéndez GG, Räisänen J, Rinke A, Sarr A, Whetton P (2007)
Regional climate projections. In: “Climate Change 2007: the Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change” (Solomon S, Qin D, Manning M, Chen Z, Marquis M, Averyt KB, Tignor M, Miller HL eds). Cambridge University press, Cambridge, UK, pp. 849-940.
Gscholar
(9)
Deslauriers A, Rossi S, Anfodillo T, Saracino A (2008)
Cambial phenology, wood formation and temperature thresholds in two contrasting years at high altitude in southern Italy. Tree Physiology 28: 863-871.
CrossRef | Gscholar
(10)
Evert RF (2006)
Esau’s plant anatomy meristems, cells, and tissues of the plant body: their structure, function, and development. John Wiley and Sons, Hoboken, USA, pp. 624.
Online | Gscholar
(11)
Fu J, Sun X, Wang J, Chu J, Yan C (2011)
Progress in quantitative analysis of plant hormones. Chinese Science Bulletin 56: 355-366.
CrossRef | Gscholar
(12)
Funada R, Kubo T, Tabuchi M, Sugiyama T, Fushitani M (2001)
Seasonal variations in endogenous indole-3-acetic acid and abscisic acid in the cambial region of Pinus densiflora stems in relation to earlywood-latewood transition and cessation of tracheid production. Holzforschung 55: 128-134.
CrossRef | Gscholar
(13)
Gregory RA (1971)
Cambial activity in Alaskan white spruce. American Journal of Botany 58: 160-171.
CrossRef | Gscholar
(14)
Gričar J, Krze L, Cufar K (2009)
Number of cells in xylem, phloem and dormant cambium in silver fir (Abies alba), in trees of different vitality. IAWA Journal 30: 121-133.
CrossRef | Gscholar
(15)
Gričar J, Prislan P, Gryc V, Vavrčík H, De Luis M, Cufar K (2014)
Plastic and locally adapted phenology in cambial seasonality and production of xylem and phloem cells in Picea abies from temperate environments. Tree Physiology 34 (8): 869-881.
CrossRef | Gscholar
(16)
Gričar J, Prislan P, De Luis M, Gryc V, Hacurová J, Vavrčík H, Cufar K (2015)
Plasticity in variation of xylem and phloem cell characteristics of norway spruce under different local conditions. Frontiers in Plant Science 6: 1-14.
CrossRef | Gscholar
(17)
Harris I, Jones PD, Osborn TJ, Lister DH (2013)
Updated high-resolution grids of monthly climatic observations - the CRU TS3.10 dataset. International Journal of Climatology 34 (3): 623-642.
CrossRef | Gscholar
(18)
Jeffs RA, Northcote DH (1966)
Experimental induction of vascular tissue in an undifferentiated plant callus. Biochemical Journal 101: 146-152.
CrossRef | Gscholar
(19)
Larson PR (1964)
Some indirect effects of environment on wood formation. In: “The formation of wood in forest tress” (Zimmermann MH ed). Academic press, New York, USA, pp. 345-365.
CrossRef | Gscholar
(20)
Larson PR (1969)
Wood formation and the concept of wood quality. Bulletin no. 74, Yale University, School of Forestry, New Haven, CT, USA, pp. 54.
Online | Gscholar
(21)
Little CH, Savidge RA (1987)
The role of plant growth regulators in forest tree cambial growth. Plant Growth Regulation 6 (1-2): 137-169.
CrossRef | Gscholar
(22)
Lupi C, Morin H, Deslauriers A, Rossi S (2010)
Xylem phenology and wood production: resolving the chicken-or-egg di lemma. Plant, Cell and Environment 33: 1721-1730.
CrossRef | Gscholar
(23)
Nakurte I, Keisa A, Rostoks N (2012)
Development and validation of a reversed-phase liquid chromatography method for the simultaneous determination of indole-3-acetic acid, indole-3-pyruvic acid, and abscisic acid in barley (Hordeum vulgare L.). Journal of Analytical Methods in Chemistry 1: 1-6.
CrossRef | Gscholar
(24)
Oribe Y, Funada R, Shibagaki M, Kubo T (2001)
Cambial reactivation in locally heated stems of evergreen conifer Abies sachalinensis (Schmidt) masters. Planta 212: 684-691.
CrossRef | Gscholar
(25)
Perrot-Rechenmann C (2010)
Cellular responses to auxin: division versus expansion. Cold Spring Harbor Perspectives in Biology 2: 1-15.
CrossRef | Gscholar
(26)
Podlešáková K, Zalabák D, Cudejková M, Plíhal O, Szüčová L, Dolezal K, Spíchal L, Strnad M, Galuszka P (2012)
Novel cytokinin derivatives do not show negative effects on root growth and proliferation in submicromolar range. PLoS ONE 7: 1-15.
CrossRef | Gscholar
(27)
Rossi S, Deslauriers A, Morin H (2003)
Application of the gompertz equation for the study of xylem cell development. Dendrochronologia 21: 33-39.
CrossRef | Gscholar
(28)
Rossi S, Anfodillo T, Menardi R (2006)
Trephor: a new tool for sampling microcores from tree stems. IAWA Journal 27: 89-97.
CrossRef | Gscholar
(29)
Savidge RA (1991)
Seasonal cambial activity in Larix laricina saplings in relation to endogenous indol-3-ylacetic acid, sucrose, and coniferin. Forest Science 37: 953-958.
Gscholar
(30)
Schrader J, Baba K, May ST, Palme K, Bennett M, Bhalerao RP, Sandberg G (2003)
Polar auxin transport in the wood-forming tissues of hybrid aspen is under simultaneous control of developmental and environmental signals. Proceedings of the National Academy of Sciences USA 100: 10096-10101.
CrossRef | Gscholar
(31)
Sheen J, Zhou L, Jang JC (1999)
Sugars as signaling molecules. Current Opinion in Plant Biology 2: 410-418.
CrossRef | Gscholar
(32)
Sieburth LE, Deyholos MK (2006)
Vascular development: the long and winding road. Current Opinion in Plant Biology 9: 48-54.
CrossRef | Gscholar
(33)
Sundberg B, Little CHA, Riding RT, Sandberg G (1987)
Levels of endogenous indole-3-acetic acid in the vascular cambium region of Abies balsamea trees during the activity-rest-quiescence transition. Physiologia Plantarum 71: 163-170.
CrossRef | Gscholar
(34)
Sundberg B, Uggla C, Tuominen H (2000)
Cambial growth and auxin gradients. In: “Cell and molecular biology of wood formation” (Savidge RA, Barnett JR, Napir R eds). Bios Scientific Publishers, Oxford, pp. 169-188.
Gscholar
(35)
Suzuki M, Yoda K, Suzuki H (1996)
Phenological comparison of the onset of vessel formation between ring-porous and diffuse-porous deciduous trees in a japanese temperate forest. IAWA Journal 17: 431-444.
CrossRef | Gscholar
(36)
Taiz L, Zeiger E (2006)
Plant Physiology (4th edn). Sinauer Associates Inc, Sunderland, UK, pp. 764.
Gscholar
(37)
Teale WD, Paponov IA, Palme K (2006)
Auxin in action: signalling, transport and the control of plant growth and development. Nature Reviews Molecular Cell Biology 7: 847-859.
CrossRef | Gscholar
(38)
Tuominen H, Puech L, Fink S, Sundberg B (1997)
A radial gradient of indole-3-acetic acid is related to secondary xylem development in hybrid aspen. Plant Physiology 115: 577-585.
CrossRef | Gscholar
(39)
Uggla C, Moritz T, Sandberg GG, Sundberg B (1996)
Auxin as a positional signal in pattern formation in plants. Proceedings of the National Academy of Sciences USA 93: 9282-9286.
CrossRef | Gscholar
(40)
Uggla C, Mellerowicz EJ, Sundberg B (1998)
Indole-3-acetic acid controls cambial growth in Scots pine by positional signaling. Plant Physiology 117: 113-121.
CrossRef | Gscholar
(41)
Uggla C, Magel E, Moritz T, Sundberg B (2001)
Function and dynamics of auxin and carbohydrates during early wood/latewood transition in Scots pine. Plant Physiology 125: 2029-2039.
CrossRef | Gscholar
(42)
Vanneste S, Friml J (2009)
Auxin: a trigger for change in plant development. Cell 136: 1005-1016.
CrossRef | Gscholar
(43)
Wodzicki TJ (1971)
Mechanisms of xylem differentiation in Pinus sylvestris L. Journal of Experimental Botany 22: 670-687.
CrossRef | Gscholar
(44)
Wetmore RH, Rier JP (1963)
Experimental induction of vascular tissues in callus of angiosperms. American Journal of Botany 50: 418-430.
CrossRef | Gscholar
 

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