iForest - Biogeosciences and Forestry


Auxin (IAA) and soluble carbohydrate seasonal dynamics monitored during xylogenesis and phloemogenesis in Scots pine

Marek Fajstavr (1-2)   , Zuzana Paschová (1), Kyriaki Giagli (1), Hanuš Vavrčík (1), Vladimír Gryc (1), Josef Urban (3-4)

iForest - Biogeosciences and Forestry, Volume 11, Issue 5, Pages 553-562 (2018)
doi: https://doi.org/10.3832/ifor2734-011
Published: Sep 01, 2018 - Copyright © 2018 SISEF

Research Articles

The metabolic activity of phytohormones and the accumulation of carbohydrates affect the reactivation of the cambial zone and the radial increment of woody plants. We aimed to monitor the dynamics of indole-3-acetic acid (IAA) concentration and amounts of soluble carbohydrates during xylem and phloem formation of one growing season (2015). Six sample trees of Scots pine (Pinus sylvestris L.), aged 80 years on average, growing in the Sobešice research site (404 m a.s.l.) in the Czech Republic were selected. We obtained microcore samples at weekly intervals by the Trephor tool method for cell formation analysis and spectrophotometric determination of IAA and soluble carbohydrate contents. We found that time of the highest concentration of IAA (last week of April) coincided with time of the maximum number of cells in the cambial zone and highest expansion of the cell enlargement stage. When the IAA concentration was too low to be measured, latewood tracheids started to form, and late phloem sieve cell formation ceased. The highest concentration of soluble carbohydrates was 200.40 ± 21.6 µg GLU per sample (May 14). This coincided with the fastest weekly xylem cell increment. This research shows that IAA and soluble carbohydrate dynamics directly affects xylem and phloem formation.


Pinus sylvestris L., Indole-3-Acetic Acid, Xylem, Phloem, Tracheids, Earlywood, Latewood

Authors’ address

Marek Fajstavr
Zuzana Paschová
Kyriaki Giagli
Hanuš Vavrčík
Vladimír Gryc
Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 3, 61300 Brno (Czech Republic)
Marek Fajstavr
The Czech Academy of Sciences, CzechGlobe - Global Change Research Institute, Institute of Plant Water Balance and Biomass Production, Belidla 4a, 60300 Brno (Czech Republic)
Josef Urban
Department of Forest Botany, Dendrology and Geobiocenology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 3, 61300 Brno (Czech Republic)
Josef Urban
Siberian Federal University, Svobodnyj Prospect 79, Krasnoyarsk, 660041 Krasnoyarsk (Russia)

Corresponding author

Marek Fajstavr


Fajstavr M, Paschová Z, Giagli K, Vavrčík H, Gryc V, Urban J (2018). Auxin (IAA) and soluble carbohydrate seasonal dynamics monitored during xylogenesis and phloemogenesis in Scots pine. iForest 11: 553-562. - doi: 10.3832/ifor2734-011

Academic Editor

Werther Guidi Nissim

Paper history

Received: Jan 19, 2018
Accepted: Jun 26, 2018

First online: Sep 01, 2018
Publication Date: Oct 31, 2018
Publication Time: 2.23 months

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List of the papers citing this article based on CrossRef Cited-by.

Abramoff MD, Magalhães PJ, Ram SJ (2004)
Image processing with imagej. Biophotonics International 11: 36-42.
Albalasmeh AA, Berhe AA, Ghezzehei TA (2013)
A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydrate Polymers 97: 253-261.
CrossRef | Gscholar
Aloni R (1995)
The induction of vascular tissues by auxin and cytokinin. In: “Plant Hormones: Physiology, Biochemistry and Molecular Biology” (Davies PJ ed). Springer, Kluwer, Dordrecht, Netherlands, pp. 531-546.
CrossRef | Gscholar
Bhalerao RP, Bennett MJ (2003)
The case for morphogens in plants. Nature Cell Biology 5: 939-943.
CrossRef | Gscholar
Bhalerao RP, Fischer U (2014)
Auxin gradients across wood - instructive or incidental? Physiologia Plantarum 151: 43-51.
CrossRef | Gscholar
Carlsbecker A, Helariutta Y (2005)
Phloem and xylem specification: pieces of the puzzle emerge. Current Opinion in Plant Biology 8: 512-517.
CrossRef | Gscholar
Denne MP (1989)
Definition of latewood according to Mork (1928). IAWA Journal 10: 59-62.
CrossRef | Gscholar
Christensen JH, Hewitson N, Busuioc A, Chen A, Gao X, Held I, Jones R, Kolli RK, Kwon WT, Laprise R, Magana Rueda V, Mearns L, Menéndez GG, Räisänen J, Rinke A, Sarr A, Whetton P (2007)
Regional climate projections. In: “Climate Change 2007: the Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change” (Solomon S, Qin D, Manning M, Chen Z, Marquis M, Averyt KB, Tignor M, Miller HL eds). Cambridge University press, Cambridge, UK, pp. 849-940.
Deslauriers A, Rossi S, Anfodillo T, Saracino A (2008)
Cambial phenology, wood formation and temperature thresholds in two contrasting years at high altitude in southern Italy. Tree Physiology 28: 863-871.
CrossRef | Gscholar
Evert RF (2006)
Esau’s plant anatomy meristems, cells, and tissues of the plant body: their structure, function, and development. John Wiley and Sons, Hoboken, USA, pp. 624.
Online | Gscholar
Fu J, Sun X, Wang J, Chu J, Yan C (2011)
Progress in quantitative analysis of plant hormones. Chinese Science Bulletin 56: 355-366.
CrossRef | Gscholar
Funada R, Kubo T, Tabuchi M, Sugiyama T, Fushitani M (2001)
Seasonal variations in endogenous indole-3-acetic acid and abscisic acid in the cambial region of Pinus densiflora stems in relation to earlywood-latewood transition and cessation of tracheid production. Holzforschung 55: 128-134.
CrossRef | Gscholar
Gregory RA (1971)
Cambial activity in Alaskan white spruce. American Journal of Botany 58: 160-171.
CrossRef | Gscholar
Gričar J, Krze L, Cufar K (2009)
Number of cells in xylem, phloem and dormant cambium in silver fir (Abies alba), in trees of different vitality. IAWA Journal 30: 121-133.
CrossRef | Gscholar
Gričar J, Prislan P, Gryc V, Vavrčík H, De Luis M, Cufar K (2014)
Plastic and locally adapted phenology in cambial seasonality and production of xylem and phloem cells in Picea abies from temperate environments. Tree Physiology 34 (8): 869-881.
CrossRef | Gscholar
Gričar J, Prislan P, De Luis M, Gryc V, Hacurová J, Vavrčík H, Cufar K (2015)
Plasticity in variation of xylem and phloem cell characteristics of norway spruce under different local conditions. Frontiers in Plant Science 6: 1-14.
CrossRef | Gscholar
Harris I, Jones PD, Osborn TJ, Lister DH (2013)
Updated high-resolution grids of monthly climatic observations - the CRU TS3.10 dataset. International Journal of Climatology 34 (3): 623-642.
CrossRef | Gscholar
Jeffs RA, Northcote DH (1966)
Experimental induction of vascular tissue in an undifferentiated plant callus. Biochemical Journal 101: 146-152.
CrossRef | Gscholar
Larson PR (1964)
Some indirect effects of environment on wood formation. In: “The formation of wood in forest tress” (Zimmermann MH ed). Academic press, New York, USA, pp. 345-365.
CrossRef | Gscholar
Larson PR (1969)
Wood formation and the concept of wood quality. Bulletin no. 74, Yale University, School of Forestry, New Haven, CT, USA, pp. 54.
Online | Gscholar
Little CH, Savidge RA (1987)
The role of plant growth regulators in forest tree cambial growth. Plant Growth Regulation 6 (1-2): 137-169.
CrossRef | Gscholar
Lupi C, Morin H, Deslauriers A, Rossi S (2010)
Xylem phenology and wood production: resolving the chicken-or-egg di lemma. Plant, Cell and Environment 33: 1721-1730.
CrossRef | Gscholar
Nakurte I, Keisa A, Rostoks N (2012)
Development and validation of a reversed-phase liquid chromatography method for the simultaneous determination of indole-3-acetic acid, indole-3-pyruvic acid, and abscisic acid in barley (Hordeum vulgare L.). Journal of Analytical Methods in Chemistry 1: 1-6.
CrossRef | Gscholar
Oribe Y, Funada R, Shibagaki M, Kubo T (2001)
Cambial reactivation in locally heated stems of evergreen conifer Abies sachalinensis (Schmidt) masters. Planta 212: 684-691.
CrossRef | Gscholar
Perrot-Rechenmann C (2010)
Cellular responses to auxin: division versus expansion. Cold Spring Harbor Perspectives in Biology 2: 1-15.
CrossRef | Gscholar
Podlešáková K, Zalabák D, Cudejková M, Plíhal O, Szüčová L, Dolezal K, Spíchal L, Strnad M, Galuszka P (2012)
Novel cytokinin derivatives do not show negative effects on root growth and proliferation in submicromolar range. PLoS ONE 7: 1-15.
CrossRef | Gscholar
Rossi S, Deslauriers A, Morin H (2003)
Application of the gompertz equation for the study of xylem cell development. Dendrochronologia 21: 33-39.
CrossRef | Gscholar
Rossi S, Anfodillo T, Menardi R (2006)
Trephor: a new tool for sampling microcores from tree stems. IAWA Journal 27: 89-97.
CrossRef | Gscholar
Savidge RA (1991)
Seasonal cambial activity in Larix laricina saplings in relation to endogenous indol-3-ylacetic acid, sucrose, and coniferin. Forest Science 37: 953-958.
Schrader J, Baba K, May ST, Palme K, Bennett M, Bhalerao RP, Sandberg G (2003)
Polar auxin transport in the wood-forming tissues of hybrid aspen is under simultaneous control of developmental and environmental signals. Proceedings of the National Academy of Sciences USA 100: 10096-10101.
CrossRef | Gscholar
Sheen J, Zhou L, Jang JC (1999)
Sugars as signaling molecules. Current Opinion in Plant Biology 2: 410-418.
CrossRef | Gscholar
Sieburth LE, Deyholos MK (2006)
Vascular development: the long and winding road. Current Opinion in Plant Biology 9: 48-54.
CrossRef | Gscholar
Sundberg B, Little CHA, Riding RT, Sandberg G (1987)
Levels of endogenous indole-3-acetic acid in the vascular cambium region of Abies balsamea trees during the activity-rest-quiescence transition. Physiologia Plantarum 71: 163-170.
CrossRef | Gscholar
Sundberg B, Uggla C, Tuominen H (2000)
Cambial growth and auxin gradients. In: “Cell and molecular biology of wood formation” (Savidge RA, Barnett JR, Napir R eds). Bios Scientific Publishers, Oxford, pp. 169-188.
Suzuki M, Yoda K, Suzuki H (1996)
Phenological comparison of the onset of vessel formation between ring-porous and diffuse-porous deciduous trees in a japanese temperate forest. IAWA Journal 17: 431-444.
CrossRef | Gscholar
Taiz L, Zeiger E (2006)
Plant Physiology (4th edn). Sinauer Associates Inc, Sunderland, UK, pp. 764.
Teale WD, Paponov IA, Palme K (2006)
Auxin in action: signalling, transport and the control of plant growth and development. Nature Reviews Molecular Cell Biology 7: 847-859.
CrossRef | Gscholar
Tuominen H, Puech L, Fink S, Sundberg B (1997)
A radial gradient of indole-3-acetic acid is related to secondary xylem development in hybrid aspen. Plant Physiology 115: 577-585.
CrossRef | Gscholar
Uggla C, Moritz T, Sandberg GG, Sundberg B (1996)
Auxin as a positional signal in pattern formation in plants. Proceedings of the National Academy of Sciences USA 93: 9282-9286.
CrossRef | Gscholar
Uggla C, Mellerowicz EJ, Sundberg B (1998)
Indole-3-acetic acid controls cambial growth in Scots pine by positional signaling. Plant Physiology 117: 113-121.
CrossRef | Gscholar
Uggla C, Magel E, Moritz T, Sundberg B (2001)
Function and dynamics of auxin and carbohydrates during early wood/latewood transition in Scots pine. Plant Physiology 125: 2029-2039.
CrossRef | Gscholar
Vanneste S, Friml J (2009)
Auxin: a trigger for change in plant development. Cell 136: 1005-1016.
CrossRef | Gscholar
Wodzicki TJ (1971)
Mechanisms of xylem differentiation in Pinus sylvestris L. Journal of Experimental Botany 22: 670-687.
CrossRef | Gscholar
Wetmore RH, Rier JP (1963)
Experimental induction of vascular tissues in callus of angiosperms. American Journal of Botany 50: 418-430.
CrossRef | Gscholar

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