Tolerance to heavy metal stress in seedlings of three pine species from contrasting environmental conditions in Chile

doi:10.3832/ifor1908-009

Tolerance to heavy metal stress in seedlings of three pine species from contrasting environmental conditions in Chile

Ariel D Arencibia , Claudio Rodríguez, Lisandro Roco, Carolina Vergara, Nataly González-Soto, Rolando García-González

iForest - Biogeosciences and Forestry, Volume 9, Issue 6, Pages 937-945 (2016)
doi: https://doi.org/10.3832/ifor1908-009
Published: Aug 12, 2016 - Copyright © 2016 SISEF

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Tolerance to metal stress was studied in seedlings of three pine species (Pinus radiata, P. pinaster and P. canariensis) under controlled ex vitro conditions. Mature female cones were randomly collected at two sites in Chile (Llico and Huilquilemu) characterized by contrasting environmental conditions. One-year-old pine seedlings were immersed in trays with solutions of CuSO₄ (300 mM) or AlCl₃ (100 mM), and their survival, growth rate and decay symptoms were recorded for 60 days. Results showed large differences among provenances in seedling tolerance to CuSO₄ and AlCl₃ in terms of survival and growth. Multivariate analysis revealed a significant association (p <0.0001) between the first canonical function and the following variables: provenance, species, metal stress and growing rate, as well as between the second canonical function and provenance, species, metal stress and symptomatology, indicating a high degree of genotype-environment interaction. Moreover, the activity of POX, SOD and CAT enzymes was determined 60 days after the beginning of the experiment in the Llico provenance seedlings. P. pinaster showed the highest activity level for all the enzymes considered, while P. canariensis and P. radiata had intermediate and lowest values, respectively. Differential gene expression among pine seedlings under metal stress with CuSO₄ for two genes (Cu-Zn-superoxide dismutase and RuBisCo) confirmed P. pinaster as the most tolerant species to CuSO₄treatment. Our results are consistent with the hypothesis that abiotic stress in the maternal environment can induce a “transgenerational plasticity” which could affect progeny performances. The influence of different genetic backgrounds on the tolerance to heavy metals in pine seedlings is also discussed.

Keywords

Pinus spp., Heavy Metals, Abiotic Stresses, Genotype-Environment Interaction

Authors’ address

(1)

Center for Biotechnology of Natural Resources, Department of Forestry, Faculty of Agronomy and Forestry Sciences, Catholic University of Maule, av.da San Miguel 3605, Talca (Chile)

Corresponding author

Ariel D Arencibia
arieldarencibia@gmail.com

Citation

Arencibia AD, Rodríguez C, Roco L, Vergara C, González-Soto N, García-González R (2016). Tolerance to heavy metal stress in seedlings of three pine species from contrasting environmental conditions in Chile. iForest 9: 937-945. - doi: 10.3832/ifor1908-009

Academic Editor

Claudia Cocozza

Paper history

Received: Nov 02, 2015
Accepted: May 02, 2016

First online: Aug 12, 2016
Publication Date: Dec 14, 2016
Publication Time: 3.40 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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