iForest - Biogeosciences and Forestry


A quick screening to assess the phytoextraction potential of cadmium and copper in Quercus pubescens plantlets

Patrick Di Santo (1), Claudia Cocozza (2)   , Roberto Tognetti (3-4), Giuseppe Palumbo (1), Erika Di Iorio (1), Bruno Paura (1)

iForest - Biogeosciences and Forestry, Volume 10, Issue 1, Pages 93-98 (2016)
doi: https://doi.org/10.3832/ifor1999-009
Published: Oct 13, 2016 - Copyright © 2016 SISEF

Short Communications

The relevance of the environmental pollution by heavy metals warrants the necessity to develop and assess more efficient plant-based technologies. This study was conducted to evaluate a quick screening approach in order to investigate the cadmium (Cd) and copper (Cu) phytoextraction potential of Quercus pubescens in a micro-propagation system. Increasing concentrations of Cd (0, 5, 50, and 250 µM) and Cu (0, 5, 50, 250 and 500 µM) were separately applied to evaluate the effect of metals on their absorption and accumulation in downy oak plants. At high concentrations, Cd and Cu significantly reduced the dry biomass of shoots and roots and the plant tolerance index. Cd was toxic at increasing concentrations, inducing higher reduction of shoot dry mass than roots, whereas Cu increased dry mass at 5 µM. This study represents the first attempt to assess Cd and Cu uptake in Q. pubescens under in vitro conditions. The in vitro screening potential is meanly related to the following purposes: (i) proper selection of plant materials resilient to excess metals in the growth substrate; (ii) efficient removal of metals by the selected tree species; (iii) minor interference with the growth of plants accumulating metals in their tissues; (iv) rapid provision of plant materials for tree breeding programs.


Heavy Metals, Phytoremediation, Downy Oak, Micropropagation

Authors’ address

Patrick Di Santo
Giuseppe Palumbo
Erika Di Iorio
Bruno Paura
Dipartimento Agricoltura, Ambiente e Alimenti, Università degli Studi del Molise, I-86100 Campobasso (Italy)
Claudia Cocozza
Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche (CNR), v. Madonna del Piano 10, I-50019 Firenze (Italy)
Roberto Tognetti
Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, I-86090 Pesche (Italy)
Roberto Tognetti
The EFI Project Centre on Mountain Forests (MOUNTFOR), Edmund Mach Foundation, I-38010 San Michele all’Adige (Italy)

Corresponding author

Claudia Cocozza


Di Santo P, Cocozza C, Tognetti R, Palumbo G, Iorio ED, Paura B (2016). A quick screening to assess the phytoextraction potential of cadmium and copper in Quercus pubescens plantlets. iForest 10: 93-98. - doi: 10.3832/ifor1999-009

Academic Editor

Tamir Klein

Paper history

Received: Jan 29, 2016
Accepted: Aug 02, 2016

First online: Oct 13, 2016
Publication Date: Feb 28, 2017
Publication Time: 2.40 months

Breakdown by View Type

(Waiting for server response...)

Article Usage

Total Article Views: 41467
(from publication date up to now)

Breakdown by View Type
HTML Page Views: 35094
Abstract Page Views: 2322
PDF Downloads: 3045
Citation/Reference Downloads: 57
XML Downloads: 949

Web Metrics
Days since publication: 2833
Overall contacts: 41467
Avg. contacts per week: 102.46

Article Citations

Article citations are based on data periodically collected from the Clarivate Web of Science web site
(last update: Feb 2023)

Total number of cites (since 2017): 3
Average cites per year: 0.43


Publication Metrics

by Dimensions ©

Articles citing this article

List of the papers citing this article based on CrossRef Cited-by.

Ali H, Khan E, Sajad MA (2013)
Phytoremediation of heavy metals - Concepts and applications. Chemosphere 91: 869-881.
CrossRef | Gscholar
Ali NA, Bernal MP, Ater M (2004)
Tolerance and bioaccumulation of cadmium by Phragmites australis grown in the presence of elevated concentrations of cadmium, copper, and zinc. Aquatic Botany 80: 163-176.
CrossRef | Gscholar
Baker AJM, Walker PL (1989)
Physiological responses of plants to heavy metals and the quantification of tolerance and toxicity. Chemical Speciation and Bioavailability 1: 7-17.
Online | Gscholar
Baldantoni D, Cicatelli A, Bellino A, Castiglione S (2014)
Different behaviours in phytoremediation capacity of two heavy metal tolerant poplar clones in relation to iron and other trace elements. Journal of Environmental Management 146: 94-99.
CrossRef | Gscholar
Capuana M (2011)
Heavy metals and woody plants - biotechnologies for phytoremediation. iForest 4: 7-15.
CrossRef | Gscholar
Cocozza C, Palumbo G, Colombo C, Tognetti R (2012)
Ecophysiological characteristics and cadmium accumulation in Downy Oak (Quercus pubescens Willd.). Forest@ - Rivista di Selvicoltura ed Ecologia Forestale 9 (5): 217-226. [in Italian with English abstract]
CrossRef | Gscholar
Confalonieri M, Balestrazzi A, Bisoffi S, Carbonera D (2003)
In vitro culture and genetic engineering of Populus spp.: synergy for forest tree improvement. Plant Cell Tissue and Organ Culture 72: 109-138.
CrossRef | Gscholar
Di Lonardo S, Capuana M, Arnetoli M, Gabbrielli R, Gonnelli C (2011)
Exploring the metal phytoremediation potential of three Populus alba L. clones using an in vitro screening. Environmental Science and Pollution Research 18: 82-90.
CrossRef | Gscholar
Doran PM (2009)
Application of plant tissue cultures in phytoremediation research: incentives and limitations. Biotechnology and Bioengineering 103: 60-76.
CrossRef | Gscholar
Fernàndez-Martínez J, Zacchini M, Fernández-Marín B, García-Plazaola JC, Fleck I (2014)
Gas-exchange, photo- and antioxidant protection, and metalaccumulation in I-214 and Eridano Populus sp. clones subjected toelevated zinc concentrations. Environmental and Experimental Botany 107: 144-153.
CrossRef | Gscholar
García-Esquinas E, Pollan M, Tellez-Plaza M, Francesconi KA, Goessler W, Guallar E, Umans JG, Yeh J, Best LG, Navas-Acien A (2014)
Cadmium exposure and cancer mortality in a prospective cohort: the Strong Heart Study. Environmental Health Perspectives 122: 363-370.
Online | Gscholar
Golan-Goldhirsh A, Barazani O, Nepovim A, Soudek P, Smrcek S, Dufkova L, Krenkova S, Yrjala K, Schröder P, Vanek T (2004)
Plant response to heavy metals and organic pollutants in cell culture and at whole plant level. Journal of Soils and Sediments 4: 133-140.
CrossRef | Gscholar
Iori V, Pietrini F, Zacchini M (2012)
Assessment of ibuprofen tolerance and removal capability in Populus nigra L. by in vitro culture. Journal of Hazardous Materials 229-230: 217-223.
CrossRef | Gscholar
Khan S, Cao Q, Zheng YM, Huang YZ, Zhu YG (2008)
Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China. Environmental Pollution 152: 686-692.
CrossRef | Gscholar
Komarek M, Tlustos P, Szakova J, Chrastny V, Ettler V (2007)
The use of maize and poplar in chelant-enhanced phytoextraction of lead from contaminated agricultural soils. Chemosphere 67: 640-651.
CrossRef | Gscholar
Kunjam M, Govada H, Mididoddi N, Kota RSLN (2015)
Studies on selected heavy metals on seed germination and plant growth in pea plant (Pisum sativum) grown in solid medium. Journal of Pharmacognosy and Phytochemistry 3 (5): 85-87.
Online | Gscholar
Lloyd G, McCown BH (1980)
Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot-tip culture. International Plant Propagation Society 30: 421-427.
Murakami M, Ae N (2009)
Potential for phytoextraction of copper, lead, and zinc by rice (Oryza sativa L.), soybean (Glycine max [L.] Merr.), and maize (Zea mays L.) Journal of Hazardous Materials 162: 1185-1192.
CrossRef | Gscholar
Paoletti E, Günthardt-Goerg MS (2006)
Growth responses and element content of Quercus pubescens seedlings under acidic and heavy metal contamination. Forest Snow and Landscape Research 3: 323-337.
Online | Gscholar
Perfus-Barbeoch L, Leonhardt N, Vavasseur A, Forestier C (2002)
Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. Plant Journal 32: 539-548.
CrossRef | Gscholar
Pietrini F, Zacchini M, Iori V, Pietrosanti L, Bianconi D, Massacci A (2010)
Screening of poplar for clones for cadmium phytoremediation using photosynthesis, biomass and cadmium content analyses. International Journal of Phytoremediation 12: 105-120.
CrossRef | Gscholar
Pilon-Smits E (2005)
Phytoremediation. Annual Review of Plant Biology 56: 15-39.
CrossRef | Gscholar
Pulford ID, Watson C (2003)
Phytoremediation of heavy metal-contaminated land by trees - A review. Environment International 29: 529-540.
CrossRef | Gscholar
Rascio N, Navari-Izzo F (2011)
Heavy metal hyperaccumulating plants: how and why do they do it? And what makes them so interesting? Plant Science 180: 169-181.
CrossRef | Gscholar
Razali NM (2011)
Power comparison of Shapiro-Wilk, Kolmogorov-Smirnov, lillefors and Anderson-Darling tests. Journal of Statistical Modeling and Analytic 2: 21-33.
Online | Gscholar
Schat H, Llugany M, Vooijs R, Hartley-Whitaker J, Bleeker PM (2002)
The role of phytochelatins in constitutive and adaptive heavy metal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes. Journal of Experimental Botany 53: 1-12.
CrossRef | Gscholar
Shah K, Nongkynrih J (2007)
Metal hyperaccumulation and bioremediation. Biologia Plantarum 51: 618-634.
CrossRef | Gscholar
Tognetti R, Cocozza C, Marchetti M (2013)
Shaping the multifunctional tree: the use of Salicaceae in environmental restoration. iForest 6: 37-47.
CrossRef | Gscholar
Wang S, Shi X, Sun H, Chen Y, Pan H, Yang X, Rafiq T (2014)
Variations in metal tolerance and accumulation in three hydroponically cultivated varieties of Salix integra treated with lead. PLoS ONE 9 (9): e108568.
CrossRef | Gscholar
Wong MH (2003)
Ecological restoration of mine degraded soils, with emphasis on metal contaminated soils. Chemosphere 50: 775-780.
CrossRef | Gscholar
Yang XE, Long XX, Ye HB, He ZL, Calvert DV, Stoffella PJ (2004)
Cadmium tolerance and hyperaccumulation in a new Zn-hyperaccumulating plant species (Sedum alfredii Hance). Plant and Soil 259: 181-189.
CrossRef | Gscholar
Zacchini M, Pietrini F, Scarascia Mugnozza G, Iori V, Pietrosanti L, Massacci A (2009)
Metal tolerance, accumulation and translocation in poplar and willow clones treated with cadmium in hydroponics. Water, Air and Soil Pollution 197: 23-34.
CrossRef | Gscholar

This website uses cookies to ensure you get the best experience on our website. More info