Identification and molecular characterization of LTR and LINE retrotransposable elements in Fagus sylvatica L.

doi:10.3832/ifor0501-002

Identification and molecular characterization of LTR and LINE retrotransposable elements in Fagus sylvatica L.

Giovanni Emiliani , Donatella Paffetti, Raffaello Giannini

iForest - Biogeosciences and Forestry, Volume 2, Issue 3, Pages 119-126 (2009)
doi: https://doi.org/10.3832/ifor0501-002
Published: Jun 10, 2009 - Copyright © 2009 SISEF

Research Articles

Collection/Special Issue: COST Action E52 Meeting 2008 - Florence (Italy)
Evaluation of beech genetic resources for sustainable forestry
Guest Editors: Georg von Wühlisch, Raffaello Giannini

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Retrotransposable elements are important and peculiar genetic components derived from ancient retrovirus insertion inside plants genome. Their ability to move and/or replicate inside the genome is an important evolutionary force, responsible for the increase of genome size and the regulation of gene expression. Retrotransposable elements are well characterized in model or crop species like Arabidopsis thaliana and Oryza sativa, but are poorly known in forest tree species. In this paper the molecular identification of retrotransposable elements in Fagus sylvatica L. is reported. Two retrotransposons, belonging to the two major classes of LTR and non-LTR elements, were characterized trough a SCAR (Sequence Characterized Amplified Region) strategy. The analysis demonstrated the presence of multiple copies of retrotransposable elements inside the genome of beech, in accordance with the viral quasi-species theory of retrotransposon evolution. The cloning and sequencing of amplification products and a Cleaved Amplified Polymorphisms (CAPs) approach on the identified retrotransposons, showed a high level of diversity among the multiple copies of both elements. The identification of retrotransposable elements in forest trees represents an important step toward the understanding of mechanisms of genome evolution. Furthermore, the high polymorphism of retrotransposable elements can represent a starting point for the development of new genetic variability markers.

Keywords

Fagus sylvatica, Retrotransposons, Genome evolution, Genetic diversity

Authors’ address

(1)

Dept. of Environmental and Forestry Technologies and Sciences, University of Florence, v. S. Bonaventura 13, I-50145 Florence (Italy).

Corresponding author

Giovanni Emiliani
giovanni.emiliani@unifi.it

Citation

Emiliani G, Paffetti D, Giannini R (2009). Identification and molecular characterization of LTR and LINE retrotransposable elements in Fagus sylvatica L.. iForest 2: 119-126. - doi: 10.3832/ifor0501-002

Paper history

Received: Feb 04, 2009
Accepted: Mar 25, 2009

First online: Jun 10, 2009
Publication Date: Jun 10, 2009
Publication Time: 2.57 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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