*
 

iForest - Biogeosciences and Forestry

*

Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants

A Zamboni   , L Pierantoni, P De Franceschi

iForest - Biogeosciences and Forestry, Volume 1, Issue 4, Pages 122-125 (2008)
doi: https://doi.org/10.3832/ifor0465-0010122
Published: Aug 27, 2008 - Copyright © 2008 SISEF

Research Articles


Studies of plant gene expression today need pure preparations of high-yielding undegraded RNA. This is not easily accomplished when working with plants and tissues like strawberry tree (Arbutus unedo) leaves that accumulate large amounts of polysaccharides and polyphenolic compounds, which co-purify with RNA. An improved leaf-tissue protocol developed for gene expression studies on Arbutus unedo yields for the first time a purity of RNA extract that makes possible cDNA synthesis and qPCR analysis in this plant species. When tested on material considered recalcitrant (leaves, roots, fruit flesh, fruit peel and styles from Pyrus communis, Prunus avium, Prunus persica and Cydonia oblonga), the method was able to extract RNA with good yield and high purity. This scalable, phenol-free, fast and easy-to-use RNA extraction protocol is effective on Arbutus unedo leaves as well as on awide range of different species and tissues, thus resulting particularly useful for gene expression analysis in non-model species for molecular biology.

  Keywords


Arbutus unedo, RNA extraction, Roots, Styles, Woody plants, Fruits

Authors’ address

(1)
A Zamboni
L Pierantoni
P De Franceschi
Department of Fruit Tree and Woody Plant Science, University of Bologna, v. Fanin 42, Bologna (Italy)

Corresponding author

 
A Zamboni
a.zamboni@unibo.it

Citation

Zamboni A, Pierantoni L, De Franceschi P (2008). Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants. iForest 1: 122-125. - doi: 10.3832/ifor0465-0010122

Academic Editor

Marco Borghetti

Paper history

Received: Feb 18, 2008
Accepted: Jun 27, 2008

First online: Aug 27, 2008
Publication Date: Aug 27, 2008
Publication Time: 2.03 months

Breakdown by View Type

(Waiting for server response...)

Article Usage

Total Article Views: 75727
(from publication date up to now)

Breakdown by View Type
HTML Page Views: 64169
Abstract Page Views: 3595
PDF Downloads: 6943
Citation/Reference Downloads: 114
XML Downloads: 906

Web Metrics
Days since publication: 5920
Overall contacts: 75727
Avg. contacts per week: 89.54

Article Citations

Article citations are based on data periodically collected from the Clarivate Web of Science web site
(last update: Feb 2023)

Total number of cites (since 2008): 33
Average cites per year: 2.06

 

Publication Metrics

by Dimensions ©

Articles citing this article

List of the papers citing this article based on CrossRef Cited-by.

 
(1)
Chaves MM, Maroco JP, Pereira JS (2003)
Understanding plant responses to drought - from genes to the whole plant. Funct. Plant. Biol. 30: 239-264.
CrossRef | Gscholar
(2)
Birtic S, Kranner I (2006)
Isolation of high-quality RNA from polyphenol-, polysaccharide- and lipid-rich seeds. Phytochem. Anal. 17 (3): 144-148.
CrossRef | Gscholar
(3)
Dal Cin V, Danesin M, Rizzini FM, Ramina A (2005)
RNA extraction from plant tissues: the use of calcium to precipitate contaminating pectic sugars. Mol. Biotechnol. 31 (2): 113-119.
CrossRef | Gscholar
(4)
De Keukeleire J, Roldán-Ruiz I, Van Bockstaele E, Heyerick A, De Keukeleire D (2006)
Efficient extraction of high-quality total RNA from various hop tissues (Humulus lupulus L.). Prep. Biochem. Biotechnol. 36 (4): 355-362.
CrossRef | Gscholar
(5)
Fang G, Hammar S, Rebecca R (1992)
A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13: 52-56.
Gscholar
(6)
Fils-Lycaon BR, Wiersma PA, Eastwell KC, Sautiere P (1996)
A cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like. Plant Physiol. 111: 269-273.
CrossRef | Gscholar
(7)
Fort F, Hayoun L, Valls J, Canals JM, Arola L, Zamora F (2008)
A new and simple method for rapid extraction and isolation of high-quality RNA from grape (Vitis vinifera) berries. J. Sci. Food Agric. 88: 179-184.
CrossRef | Gscholar
(8)
Kiefer E, Heller W, Ernst D (2000)
A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites. Plant Mol. Biol. Rep. 18: 33-39.
CrossRef | Gscholar
(9)
Komjanc M, Festi S, Rizzotti L, Cattivelli L, Cervone F, De Lorenzo G (1999)
A leucine-rich repeat receptor-like protein kinase (LRPKm1) gene is induced in Malus x domestica by Venturia inaequalis infection and salicylic acid treatment. Plant Mol. Biol. 40: 945-957.
CrossRef | Gscholar
(10)
Le Provost G, Herrera R, Paiva JA, Chaumeil P, Salin F, Plomion C (2007)
A micromethod for high throughput RNA extraction in forest trees. Biol. Res. 40 (3): 291-297.
Online | Gscholar
(11)
Li H-Y, Gray JE (1997)
Pollination-enhanced expression of a receptor-like protein kinase related gene in tobacco styles. Plant Mol. Biol. 33: 653-665.
CrossRef | Gscholar
(12)
MacLean DD, Murr DP, DeEll JR, Mackay AB, Kupferman EM (1996)
Inhibition of PAL, CHS, and ERS1 in “Red d’Anjou” pear (Pyrus communis L.) by 1-MCP. Postharvest Biol. Technol. 45: 46-55.
CrossRef | Gscholar
(13)
Malnoy M, Reynoird JP, Mourgues F, Chevreau E, Simoneau P (2001)
A method for isolating total RNA from pear leaves. Plant Mol. Biol. Rep. 19: 69a-69f.
CrossRef | Gscholar
(14)
Manning K (1991)
Isolation of nucleic acid from plants by differential solvent precipitation. Anal. Biochem. 195: 45-50.
CrossRef | Gscholar
(15)
Meisel L, Fonseca B, González S, Baezayates R, Cambiazo V, Campos R, Gonzalez M, Orellana A, Retamales J, Silva H (2005)
A rapid and efficient method for purifying high quality total RNA from peaches (Prunus persica) for functional genomics analyses. Biol. Res. 38: 83-88.
Online | Gscholar
(16)
Salzman RA, Fujita T, Zhu-Salzman K, Hasegawa PM, Bressan RA (1999)
An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol. Biol. Rep. 17:11-17.
CrossRef | Gscholar
(17)
Sambrook J, Fitsch EF, Maniatis T (1989)
Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, MA, USA.
Gscholar
(18)
Sanchez AM, Mariani C (2002)
Expression of the ACC synthase and ACC oxidase coding genes after self-pollination and incongruous pollination of tobacco pistils. Plant Mol. Biol. 48: 351-359.
CrossRef | Gscholar
(19)
Suzuki Y, Hibino T, Kawazu T, Wada T, Kihara T, Koyama H (2003)
Extraction of total RNA from leaves of eucalyptus and other woody and herbaceous plants using sodium isoascorbate. Biotechniques 34 (5): 988-990, 992-993.
Online | Gscholar
(20)
Wan CY, Wilkins TA (1994)
A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Anal. Biochem. 233 (1): 7-12.
CrossRef | Gscholar
(21)
Wang X, Tian W, Li Y (2008)
Development of an efficient protocol of RNA isolation from recalcitrant tree tissues. Mol. Biotechnol. 38 (1): 57-64.
CrossRef | Gscholar
(22)
Winkel-Shirley B (2002)
Biosynthesis of flavonoids and effects of stress. Curr. Opinion Plant. Biol. 5: 218-223.
CrossRef | Gscholar
 

This website uses cookies to ensure you get the best experience on our website. More info