Fertilisation of Quercus seedlings inoculated with Tuber melanosporum: effects on growth and mycorrhization of two host species and two inoculation methods

doi:10.3832/ifor2096-009

Fertilisation of Quercus seedlings inoculated with Tuber melanosporum: effects on growth and mycorrhization of two host species and two inoculation methods

Sergi Garcia-Barreda^(1-2) , Sara Molina-Grau⁽³⁾, Santiago Reyna⁽⁴⁾

iForest - Biogeosciences and Forestry, Volume 10, Issue 1, Pages 267-272 (2016)
doi: https://doi.org/10.3832/ifor2096-009
Published: Dec 13, 2016 - Copyright © 2016 SISEF

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Modern truffle cultivation is based on use of inoculated seedlings, which should exhibit highly colonised roots as well as a vegetative quality enhancing field plant performance. However, poor shoot and fine root growth has been a frequent issue in inoculated Quercus seedlings production. Fertilisation is a common solution in forest nurseries, but high fertilisation levels have been found to inhibit the formation of ectomycorrhizas of many fungal species. The influence of slow-release fertilisation (52 mg N, 26 mg P and 36 mg K per seedling) on growth and ectomycorrhizal status of Tuber melanosporum-inoculated seedlings was evaluated. Host species Quercus ilex and Quercus faginea and inoculation methods involving root-dipping and root-powdering were tested. Fertilisation increased weight of both host species without significant detrimental effects on ectomycorrhizal colonisation, showing that it can be effectively used in inoculated seedlings production. Both host species showed similar response to fertilisation. The inoculation method affected seedling weight and ectomycorrhizal status, suggesting that some inoculant carriers are able to damage Quercus development and T. melanosporum colonisation. The study provided an important basis for fine-tuning the use of fertilisers in truffle-inoculated seedling production.

Keywords

Fertilisation, Seedling, Nursery, Ectomycorrhizal, Inoculation

Authors’ address

(1)

Unidad de Recursos Forestales, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza (Spain)

(2)

Centro de Investigación y Experimentación en Truficultura de la Diputación de Huesca, Polígono Fabardo s/n, 22430 Graus (Spain)

(3)

Fundación Centro de Estudios Ambientales del Mediterráneo, C/ Charles Darwin 14 Parque Tecnológico, 46980 Paterna (Spain)

(4)

ETS Ingeniería Agronómica y del Medio Natural, Universitat Politècnica de València, Camino de Vera s/n, 46021 Valencia (Spain)

Corresponding author

Sergi Garcia-Barreda
sergigarciabarreda@gmail.com

Citation

Garcia-Barreda S, Molina-Grau S, Reyna S (2016). Fertilisation of Quercus seedlings inoculated with Tuber melanosporum: effects on growth and mycorrhization of two host species and two inoculation methods. iForest 10: 267-272. - doi: 10.3832/ifor2096-009

Academic Editor

Andrea Cutini

Paper history

Received: Apr 27, 2016
Accepted: Aug 22, 2016

First online: Dec 13, 2016
Publication Date: Feb 28, 2017
Publication Time: 3.77 months

Open Access

This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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List of the papers citing this article based on CrossRef Cited-by.

(1)

Andres-Alpuente A, Sanchez S, Martin M, Aguirre AJ, Barriuso JJ (2014)
Comparative analysis of different methods for evaluating quality of Quercus ilex seedlings inoculated with Tuber melanosporum. Mycorrhiza 24: S29-S37.
CrossRef | Gscholar

(2)

Averseng P, Rouch P (2001)
Quatre étapes de l’amélioration d’un produit au travers d’un quart de siècle de coopération entre l’INRA et Agri-Truffe [Four steps for the improvement of a product in a quarter of century of cooperation between INRA and Agri-Truffe]. In: Proceedings of the "Vè Congrès International Science et Culture de la Truffe" (Sourzat P ed). Aix-en-Provence (France), 4-6 March 1999. Fédération Française des Trufficulteurs, Marseille, France, pp. 293-295. [in French]
Gscholar

(3)

Beckjord PR, Melhuish Jr JH, McIntosh MS (1985)
Effects of nitrogen and phosphorus fertilization on growth and formation of ectomycorrhizae of Quercus alba and Q. rubra seedlings by Pisolithus tinctorius and Scleroderma auranteum. Canadian Journal of Botany 63: 1677-1680.
Online | Gscholar

(4)

Benucci GMN, Gógán Csorbai A, Baciarelli Falini L, Bencivenga M, Di Massimo G, Donnini D (2012)
Mycorrhization of Quercus robur L., Quercus cerris L. and Corylus avellana L. seedlings with Tuber macrosporum Vittad. Mycorrhiza 22: 639-646.
CrossRef | Gscholar

(5)

Bourrières D, Coves H, Tixier R, Ricard JM (2005)
Effects of the initial level of mycorrhization of young plants inoculated with Tuber melanosporum. In: Abstracts book of “IV International Workshop on Edible Mycorrhizal Mushrooms (IWEMM4)”. Murcia (Spain) 28 Nov - 2 Dec 2005. Universidad de Murcia, Murcia, Spain, pp. 32.
Gscholar

(6)

Cartié G, Palazón C, Barriuso JJ, Delgado I (1996)
Un nuevo método de inoculación de Quercus ilex L. por Tuber melanosporum Vitt. [A new method of Quercus ilex L. inoculation by Tuber melanosporum Vitt.]. Revista Montes 46: 40-43. [in Spanish]
Gscholar

(7)

Cartié G, Palazón C, Delgado I, Barriuso JJ (2001)
Influencia del método de inoculación, del tipo de substrato y de la procedencia de la trufa, en la micorrización de Quercus ilex L. por Tuber melanosporum Vitt. y en la supervivencia de las plantas [Influence of inoculation method, kind of substrate and origin of truffle on Quercus ilex L. mycorrhization by Tuber melanosporum Vitt. and plant survival]. In: Proceedings of the “Vè Congrès International Science et Culture de la Truffe” (Sourzat P ed). Aix-en-Provence (France) 4-6 March 1999. Fédération Française des Trufficulteurs, Marseille, France, pp 296-299. [in Spanish]
Gscholar

(8)

Castellano MA, Molina R (1989)
Mycorrhizae. In: “The Container Tree Nursery Manual, Vol. 5. The biological component: nursery pests and mycorrhizae” (Landis TD, Tinus RW, McDonald SE, Barnett JP eds). USDA Forest Service, Washington, USA, pp. 101-171.
Gscholar

(9)

Chevalier G, Grente J (1978)
Application pratique de la symbiose ectomycorhizienne: production a grande echelle de plants mycorhizes par la truffe (Tuber melanosporum Vitt.) [Practical application of ectomycorrhizal symbiosis: large-scale production of plants mycorrhized by truffle]. Mushroom Science 10: 483-505.
Gscholar

(10)

Cortina J, Vilagrosa A, Trubat R (2013)
The role of nutrients for improving seedling quality in drylands. New Forests 44: 719-732.
CrossRef | Gscholar

(11)

Diaz G, Carrillo C, Honrubia M (2010)
Mycorrhization, growth and nutrition of Pinus halepensis seedlings fertilized with different doses and sources of nitrogen. Annals of Forest Science 67: 405.
CrossRef | Gscholar

(12)

Domínguez-Núñez JA, Selva-Serrano J, Rodríguez-Barreal JA, Saiz de Omeñaca JA (2006)
The influence of mycorrhization with Tuber melanosporum in the afforestation of a Mediterranean site with Quercus ilex and Quercus faginea. Forest Ecology and Management 231: 226-233.
CrossRef | Gscholar

(13)

Dupré C, Chevalier G, Morizet J, Leblevenec L (1982)
Influence de l’azote et du phosphore sur la mycorhization de Quercus pubescens Willd. par Tuber melanosporum Vitt. en conditions contrôlées [Influence of nitrogen and phosphorus on mycorrhiza formation by Quercus pubescens Willd. and Tuber melanosporum Vitt. under controlled conditions]. In: “Les mycorhizes, partie intégrante de la plante: biologie et perspectives d’utilisation”. (Gianinazzi S, Gianinazzi-Pearson V, Trouvelot A eds). INRA seminar no. 13, INRA, Dijon, France, pp. 147-154. [in French]
Gscholar

(14)

Fischer CR, Colinas C (1996)
Methodology for certification of Quercus ilex seedlings inoculated with Tuber melanosporum for commercial application. In: Abstracts booklet of “1^st International Conference on Mycorrhizae (ICOM1)” (Szaro TM, Bruns TD eds). Berkeley (California, USA) 4-9 Aug 1996. University of California, Berkeley, California, USA, p 34.
Gscholar

(15)

Garcia-Barreda S, Reyna S (2013)
Cultivation of Tuber melanosporum in firebreaks: short-term persistence of the fungus and effect of seedling age and soil treatment. Fungal Biology 117: 783-790.
CrossRef | Gscholar

(16)

Garcia-Barreda S, Reyna S, Pérez-Badía R, Rodríguez-Barreal JA, Domínguez-Núñez JA (2007)
Ecología de la trufa y las áreas truferas [Ecology of truffle and truffle areas]. In: “Truficultura: Fundamentos y Técnicas” (Reyna S ed). Ed. Mundi-Prensa, Madrid, Spain, pp. 153-208. [in Spanish]
Gscholar

(17)

Hall I, Brown GT, Zambonelli A (2007)
Taming the truffle. Timber Press, Portland, USA, pp. 304.
Gscholar

(18)

Khasa PD, Sigler L, Chakravarty P, Dancik BP, Erickson L, McCurdy D (2001)
Effect of fertilization on growth and ectomycorrhizal development of container-grown and bare-root nursery conifer seedlings. New Forests 22: 179-197.
CrossRef | Gscholar

(19)

Murat C (2015)
Forty years of inoculating seedlings with truffle fungi: past and future perspectives. Mycorrhiza 25: 77-81.
CrossRef | Gscholar

(20)

Navarro FB, Jiménez N, Ripoll MA, Fernández-Ondoño E, Gallego E, De Simón E (2006)
Direct sowing of holm oak acorns: effects of acorn size and soil treatment. Annals of Forest Science 63: 961-967.
CrossRef | Gscholar

(21)

Oliveira RS, Franco AR, Vosátka M, Castro PML (2010)
Management of nursery practices for efficient ectomycorrhizal fungi application in the production of Quercus ilex. Symbiosis 52: 125-131.
CrossRef | Gscholar

(22)

Palazón C, Barriuso JJ (2007)
Viveros y producción de planta micorrizada [Nurseries and production of mycorrhizal plants]. In: “Truficultura: Fundamentos y técnicas” [Trufficulture: Principles and Techniques] (Reyna S ed). Ed. Mundi-Prensa, Madrid, Spain, pp. 209-236.
Gscholar

(23)

Pruett GE, Bruhn JN, Mihail JD (2008)
Colonization of Pedunculate oak by the Burgundy truffle fungus is greater with natural than with pelletized lime. Agroforesty Systems 72: 41-50.
CrossRef | Gscholar

(24)

Quoreshi M, Timmer VR (2000)
Growth, nutrient dynamics, and ectomycorrhizal development of container-grown Picea mariana seedlings in response to exponential nutrient loading. Canadian Journal of Forest Research 30: 191-201.
CrossRef | Gscholar

(25)

Reyna S, Garcia-Barreda S (2014)
Black truffle cultivation: a global reality. Forest Systems 23: 317-328.
CrossRef | Gscholar

(26)

Rincon A, Parlade J, Pera J (2007)
Influence of the fertilisation method in controlled ectomycorrhizal inoculation of two Mediterranean pines. Annals of Forest Science 64: 577-583.
CrossRef | Gscholar

(27)

Sánchez S, Gómez E, Martín M, De Miguel AM, Urban A, Barriuso J (2014)
Experiments on the life cycle and factors affecting reproduction of Sphaerosporella brunnea provide evidence for rapid asexual propagation by conidiospores and for homothallism in an ectomycorrhizal competitor of cultivated truffle species. Fungal Ecology 8: 59-65.
CrossRef | Gscholar

(28)

Sanz-Pérez V, Castro-Díez P, Valladares F (2007)
Growth versus storage: responses of Mediterranean oak seedlings to changes in nutrient and water availabilities. Annals of Forest Science 64: 201-210.
CrossRef | Gscholar

(29)

Silla F, Escudero A (2004)
Nitrogen-use efficiency: trade-offs between N productivity and mean residence time at organ, plant and population levels. Functional Ecology 18: 511-521.
CrossRef | Gscholar

(30)

Sourzat P (2008)
Principe de précaution en trufficulture [The principle of precaution in truffle cultivation]. Station d’Expérimentation sur la Truffe, Le Montat, France, pp 72. [in French]
Gscholar

(31)

Tennant D (1975)
A test of a modified line intersect method of estimating root length. Journal of Ecology 63: 995-1001.
CrossRef | Gscholar

(32)

Treseder KK (2004)
A meta-analysis of mycorrhizal responses to nitrogen, phosphorus, and atmospheric CO₂ in field studies. New Phytologist 164: 347-355.
CrossRef | Gscholar

(33)

Uscola M, Salifu KF, Oliet JA, Jacobs DF (2015)
An exponential fertilization dose-response model to promote restoration of the Mediterranean oak Quercus ilex. New Forests 46: 795-812.
CrossRef | Gscholar

(34)

Villar-Salvador P, Planelles R, Enríquez E, Peñuelas-Rubira J (2004)
Nursery cultivation regimes, plant functional attributes, and field performance relationships in the Mediterranean oak Quercus ilex L. Forest Ecology and Management 196: 257-266.
CrossRef | Gscholar

(35)

Villar-Salvador P, Nicolás-Peragón JL, Heredia-Guerrero N, Uscola M (2012a)
Quercus ilex L. In: “Producción y manejo de semillas y plantas forestales. Tomo II” [Production and handling of forest seeds and seedlings. Vol. II] (Pemán J, Navarro-Cerrillo RM, Nicolás-Peragón JL, Prada-Sáez MA, Serrada R eds). Organismo Autónomo Parques Nacionales, Ministerio de Agricultura, Alimentación y Medio Ambiente, Madrid, Spain, pp. 226-250. [in Spanish]
Gscholar

(36)

Villar-Salvador P, Oliet J, Heredia-Guerrero N, Uscola-M, Goicoetxea P (2012b)
Quercus faginea Lam. - Quercus humilis Mill. In: “Producción y manejo de semillas y plantas forestales. Tomo II” [Production and handling of forest seeds and seedlings. Vol. II] (Pemán J, Navarro-Cerrillo RM, Nicolás-Peragón JL, Prada-Sáez MA, Serrada R eds). Organismo Autónomo Parques Nacionales, Ministerio de Agricultura, Alimentación y Medio Ambiente, Madrid, Spain, pp. 206-225. [in Spanish]
Gscholar

(37)

Walker RF, Walker RF, McLaughlin SB, Amundsen CC (2003)
Interactive effects of mycorrhization and fertilization on growth, nutrition, and water relations of sweet birch. Journal of Sustainable Forestry 17: 55-80.
CrossRef | Gscholar

(38)

Wallander H (1995)
A new hypothesis to explain allocation of dry matter between mycorrhizal fungi and seedlings in relation to nutrient supply. Plant and Soil 168-169: 243-248.
CrossRef | Gscholar

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