iForest - Biogeosciences and Forestry


Fertilisation of Quercus seedlings inoculated with Tuber melanosporum: effects on growth and mycorrhization of two host species and two inoculation methods

Sergi Garcia-Barreda (1-2)   , Sara Molina-Grau (3), Santiago Reyna (4)

iForest - Biogeosciences and Forestry, Volume 10, Issue 1, Pages 267-272 (2016)
doi: https://doi.org/10.3832/ifor2096-009
Published: Dec 13, 2016 - Copyright © 2016 SISEF

Research Articles

Modern truffle cultivation is based on use of inoculated seedlings, which should exhibit highly colonised roots as well as a vegetative quality enhancing field plant performance. However, poor shoot and fine root growth has been a frequent issue in inoculated Quercus seedlings production. Fertilisation is a common solution in forest nurseries, but high fertilisation levels have been found to inhibit the formation of ectomycorrhizas of many fungal species. The influence of slow-release fertilisation (52 mg N, 26 mg P and 36 mg K per seedling) on growth and ectomycorrhizal status of Tuber melanosporum-inoculated seedlings was evaluated. Host species Quercus ilex and Quercus faginea and inoculation methods involving root-dipping and root-powdering were tested. Fertilisation increased weight of both host species without significant detrimental effects on ectomycorrhizal colonisation, showing that it can be effectively used in inoculated seedlings production. Both host species showed similar response to fertilisation. The inoculation method affected seedling weight and ectomycorrhizal status, suggesting that some inoculant carriers are able to damage Quercus development and T. melanosporum colonisation. The study provided an important basis for fine-tuning the use of fertilisers in truffle-inoculated seedling production.


Fertilisation, Seedling, Nursery, Ectomycorrhizal, Inoculation

Authors’ address

Sergi Garcia-Barreda
Unidad de Recursos Forestales, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza (Spain)
Sergi Garcia-Barreda
Centro de Investigación y Experimentación en Truficultura de la Diputación de Huesca, Polígono Fabardo s/n, 22430 Graus (Spain)
Sara Molina-Grau
Fundación Centro de Estudios Ambientales del Mediterráneo, C/ Charles Darwin 14 Parque Tecnológico, 46980 Paterna (Spain)
Santiago Reyna
ETS Ingeniería Agronómica y del Medio Natural, Universitat Politècnica de València, Camino de Vera s/n, 46021 Valencia (Spain)

Corresponding author

Sergi Garcia-Barreda


Garcia-Barreda S, Molina-Grau S, Reyna S (2016). Fertilisation of Quercus seedlings inoculated with Tuber melanosporum: effects on growth and mycorrhization of two host species and two inoculation methods. iForest 10: 267-272. - doi: 10.3832/ifor2096-009

Academic Editor

Andrea Cutini

Paper history

Received: Apr 27, 2016
Accepted: Aug 22, 2016

First online: Dec 13, 2016
Publication Date: Feb 28, 2017
Publication Time: 3.77 months

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