iForest - Biogeosciences and Forestry


Detection and quantification of the air inoculum of Caliciopsis pinea in a plantation of Pinus radiata in Italy

Leticia Botella (1)   , Aneta Bačová (1), Milon Dvorák (2), Tomáš Kudláček (1), Alessia L Pepori (3), Alberto Santini (3), Luisa Ghelardini (3), Nicola Luchi (3)

iForest - Biogeosciences and Forestry, Volume 12, Issue 2, Pages 193-198 (2019)
doi: https://doi.org/10.3832/ifor2866-012
Published: Apr 10, 2019 - Copyright © 2019 SISEF

Short Communications

Caliciopsis pinea has been historically described as a secondary pathogen of pines. However, it has recently been associated with severe damages on Pinus radiata in Italy. Our study focused on the description of the seasonal spore dispersal of C. pinea and its relation to meteorological conditions (temperature, leaf wetness, relative humidity and precipitations). For this experiment one infected P. radiata plantation was sampled in Tuscany (Italy). A rotating arm spore trap together with a weather station were installed to sample the aerospora for 24 h every week from May to November 2016. Exposed tapes from spore traps were directly analyzed after DNA extraction by qPCR using specific primers and TaqMan MGB probe. The study shows an irregular occurrence of the inoculum of C. pinea throughout the whole sampling period with peak levels in mid-June and early August. The statistical analysis of the DNA and climatic data clearly show the strong influence of precipitation on the spore production of this pathogen. Furthermore, the very low detection limit of the qPCR experiment shows the efficacy and suitability of rotating arm spore traps for early detection of this pathogen.


Caliciopsis Canker, Monterey Pine, Aerospora, qPCR

Authors’ address

Leticia Botella 0000-0002-6613-5405
Aneta Bačová
Tomáš Kudláček
Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 1, 613 00 Brno (Czech Republic)
Milon Dvorák
Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelská 1, 613 00 Brno (Czech Republic)
Alessia L Pepori
Alberto Santini 0000-0002-7955-9207
Luisa Ghelardini 0000-0002-3180-4226
Nicola Luchi 0000-0003-3119-7574
Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), v. Madonna del Piano 10, 50019 Sesto Fiorentino, Firenze (Italy)

Corresponding author

Leticia Botella


Botella L, Bačová A, Dvorák M, Kudláček T, Pepori AL, Santini A, Ghelardini L, Luchi N (2019). Detection and quantification of the air inoculum of Caliciopsis pinea in a plantation of Pinus radiata in Italy. iForest 12: 193-198. - doi: 10.3832/ifor2866-012

Academic Editor

Claudia Cocozza

Paper history

Received: May 24, 2018
Accepted: Jan 21, 2019

First online: Apr 10, 2019
Publication Date: Apr 30, 2019
Publication Time: 2.63 months

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