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iForest - Biogeosciences and Forestry
vol. 7, pp. 282-288
Copyright © 2014 by the Italian Society of Silviculture and Forest Ecology
doi: 10.3832/ifor1076-007

Research Articles

Relationship between frost hardiness of adults and seedlings of different tree species

Maria Hofmann (1)Corresponding author, Michael Jager (1), Helge Bruelheide (1-2)

Introduction 

Out of all factors worldwide that potentially limit geographical distribution ranges of plants, minimum temperatures are considered the most important ([39], [12], [17]). In particular, frost is thought to be the cause of northern and eastern distribution range boundaries of plant species in Europe ([33], [29], [22]). The coincidence between species distribution boundaries and freezing temperature thresholds has been already demonstrated for various plant species ([33], [19], [40]). For example, Fagus sylvatica L. does not occur in regions with a mean temperature in January below -3 °C ([8]). However, these observations are purely based on correlations and knowledge on which plant organs are affec- ted by frost in which phenological state is virtually absent. Low temperatures affect individuals directly or indirectly by frost dehydration ([28], [5]), whereby single cells up to the whole organism can be damaged ([38], [28]). Thus, tree species in the northern hemisphere have evolved a certain frost hardiness despite the high energetic costs involved ([18]). Frost hardiness of European plant species shows a seasonal pattern and at the end of the growing season it is induced by decreasing temperatures ([41], [31], [5]) and is brought about by accumulation of carbohydrates and dehydration of the cells, tissues and organs ([34], [33], [2], [37], [26], [10]).

Long-lived plant species go through various development stages with different environmental condition requirements. It is well-known that frost hardiness varies along the plant life cycle ([33], [7], [36]), with seedlings exhibiting a lower frost hardiness than adults. For example, Morin et al. ([26]) showed for different European Quercus species that adults were significantly more frost-tolerant than seedlings. However, despite such differences in frost tolerance between adults and juveniles, one would expect that frost tolerance mechanisms of different ontogenetic stages are similar for a given species. Moreover, seedlings and adults might not share the same micro-environment but are exposed to the same macro-climate at any growth location. In consequence, the survival and growth of seedlings of the tree species Acer pictum subsp. mono (Maximowicz) H. Ohashi and Fagus crenata Blume were found to be in accordance with the distribution patterns of the adult individuals of these species in Japan ([25]).

Periods of low temperatures are critical events in the development of tree seedlings. While seeds of temperate tree species are generally frost-tolerant down to -196 °C ([33]), they lose frost hardiness with the onset of germination ([24]). As most temperate and boreal tree species germinate at the beginning of the growing season in spring, they are often exposed to late frost events which frequently occur in the temperate and boreal zone ([7]), and therefore, frost events can severely affect seedling growth and survival ([7], [23]). For plant species in an alpine glacier foreland, Marcante et al. ([24]) showed that freezing temperatures in the growing season are potentially severe enough to kill plants at early stages of their development. Similarly, Funkenberg et al. ([14]) demonstrated the importance of late frosts for establishment of Fallopia japonica (Houtt.) Ronse Decr. seedlings in Germany, whereby the freezing sensitivity depended on the timing, intensity and duration of late frost events. The fact that trees often can be grown in Botanical Gardens outside the species’ native distribution range when planted as saplings, indicates that tree species distribution ranges might depend more on the susceptibility of seedlings rather than that of adults. Furthermore, frost hardiness of seedlings depends on their developmental stage. There is evidence that frost tolerance decreases with increasing seedling age as demonstrated by Bigras et al. ([7]) for seedlings of Picea glauca (Moench) Voss.

In this study, we asked to which degree frost tolerance of adult individuals and seedlings of eight temperate tree species common to Central Europe corresponds to their geographical distribution range. We tested the hypothesis that the more tolerant to frost is a species the further its distribution range extends to regions with low winter temperatures.

In particular, we expected the species’ geographical distribution is better explained by the frost tolerance of seedlings than that of adult individuals. Furthermore, we tested the hypothesis that the frost hardiness of seedlings increases with increasing developmental stage.

Materials and Methods 

Experimental design

We investigated individuals of the following eight tree species: Acer negundo L., Acer platanoides L., Alnus glutinosa (L.) P. Gaertn., Betula pendula Roth, Fagus sylvatica L., Picea abies (L.) H. Karst., Pseudotsuga menziesii (Mirb.) Franco and Tilia cordata Mill. For all species we investigated four different development stages: adult trees, one-week, two-weeks and four-weeks old seedlings. While in the one-week old seedlings the first leaves (either the cotyledons or the primary leaves) were just unfolded, they were developed in two-weeks old seedlings and subsequent leaves already emerged in the four-week old seedlings (Fig. 1).

Fig. 1 - Developmental stages of one-week (A, D), two-weeks (B, E) and four-weeks old seedlings (C, F) of Acer platanoides (A-C) and Pseudotsuga menziesii (D-F).

Frost hardiness of the adult individuals was analyzed on winter buds (A. negundo, A. platanoides, A. glutinosa, B. pendula, F. sylvatica and T. cordata) or needles (P. abies and P. menziesii). Except for A. negundo, which is highly invasive in Central Germany, and P. menziesii, which is an important plantation species, all species were native to the study region in the surrounding of Halle (Saale), Germany (latitude: N 51.497°, longitude E 11.969°, elevation 87 m a.s.l., annual mean temp: 9.0 °C, annual precipitation: 490 mm). Plant material was sampled about 12 hours before the start of the frost experiment and stored at +4 °C.

Frost hardiness of seedlings was analyzed on whole individuals. Seedlings derived from seeds collected from natural stands in Saxony-Anhalt (Landesdarre Saxony-Anhalt, Annaburg - Tab. 1). In early spring 2012 the seeds were sown in pots containing compost and sand (2:1 v/v) in a glasshouse chamber (day/night cycle 12 h, 20 °C / 10 °C). Because of the low germination rates of F. sylvatica and T. cordata, further seedlings were collected in the field in April 2012. The seedlings of every development stage were acclimated in a climate chamber (RUMED, day/night cycle 12 h, 8 °C / 4 °C ± 0.6 °C temperature, lamps from ISTA, 35000 Lumen) one week prior to the frost experiment to simulate outdoor conditions.

Tab. 1 - Geographic origin (in decimal degrees) of the seed material of the investigated species.

Frost experiment

Frost hardiness was tested in a climate test chamber (SANYO Atmos Chamber MTH-4400), using the procedure described in Hofmann et al. ([16]). Frost hardiness of the adult species was determined in winter 2010/ 2011 (A. negundo, A. platanoides, A. glutinosa, F. sylvatica, T. cordata) and 2011/ 2012 (B. pendula, P. abies, P. menziesii), using one to seven individuals per species (A. negundo: n = 6, A. platanoides: n = 5, A. glutinosa: n = 1, B. pendula: n = 1, F. sylvatica: n = 3, P. abies: n = 1, P. menziesii: n = 1, T. cordata: n = 7). Frost hardiness of the seedlings was determined in spring 2012. Briefly, the plant material was exposed stepwise to 11 temperature levels for buds of adult individuals: +4 °C, -4 °C, -8 °C, -12 °C, -16 °C, -20 °C, -24 °C, -28 °C, -32 °C, -40 °C and -80 °C; for seedlings: +4 °C, 0 °C, -2 °C, -4 °C, -6 °C, -8 °C, -10 °C, -14 °C, -18 °C, -22 °C and -40 °C with a cooling rate of 0.13 and 0.07 °C / min between frost levels for adults and seedlings, respectively. Each temperature level lasted for 45 minutes (resulting in 8.15 hours in total). At the end of each level, one batch of winter buds from adult trees and eight pots with seedlings were removed from the climate test chamber and transferred to a refrigerator at +4 °C to allow for controlled thawing and stored there until the next day. Eight replicates per treatment, resulting in a total of 88 replicates per developmental stage and species were considered.

Assessment of frost hardiness of adult individuals

Frost hardiness of the adult individuals was measured as the rate of electrolyte leakage of the buds. On the next day after frost exposure, buds were transferred into test tubes with 3%-isopropanol solution and tested for electrolyte leakage according to Murray et al. ([27]). Intracellular ice formation results in cell membrane disruption, whereby protoplasm escapes, resulting in an increase of the electrolyte conductivity in the surrounding solution ([3]). The electrical conductivity in the solution was measured six times: first immediately after preparing the buds to define a baseline for electrical conductivity (C0), followed by four measurements after 4 h, 24 h, 48 h and 72 h (Ct) after the initial measurement. A final measurement was conducted after boiling the samples for 20 minutes (Cb), which resulted in a complete destruction of the tissue and served as the maximum electrical conductivity of the plant material. Relative Conductivity (RC) was calculated according to eqn. 1 ([27]):

\begin{equation} RC = \frac{C_{t} - C_0}{C_{b} - C_0} = 1 - e^{-k \cdot t} \end{equation}

The rate of electrolyte leakage (k-values) was calculated for all samples (n = 88) per species and development stage, by a 4-parametric sigmoid regression according to eqn. 2:

\begin{equation} k = f(T) = c+ {\frac{a} {1 + e^{- \left ( \frac{T - LT_{50}} {b} \right )}}} \end{equation}

where a is the range of k-values, b is the steepness of the curve, c is the minimum k-values, T is the temperature. The regression parameter LT50 describes the point of inflection of the resulting curve and indicates the temperature at which 50% of maximum electrolyte leakage was reached.

Assessment of frost hardiness of seedlings

Frost hardiness of the seedlings was measured by visual inspection of the seedlings’ survival. On the following day of the frost experiment the seedlings were transferred into a glasshouse chamber (day/night cycle 12 h, 20 °C / 10 °C). Two days later the survival of every individual seedling was assessed. Survival was then regressed on the temperature levels applied in a logistic model and LT50 was obtained from eqn. 3:

\begin{equation} \ln \left ( \frac{p}{1-p} \right ) = f(T) = aT + b \end{equation}

where p is the proportion of seedlings that survived, a and b are regression parameters, T is the temperature.

The point of inflection of the above function is the LT50, i.e., the temperature at which 50% of the seedlings survived, and is given as -b/a. A pre-study demonstrated that the LT50-values obtained by electrolyte leakage were comparable to the LT50-values calculated by survival rates. These results show that frost hardiness of seedlings assessed by visual inspection and electrolyte leakage was closely related (Fig. 2).

Fig. 2 - Comparison of the frost hardiness measurements for four-weeks old seedlings of Tilia cordata. Solid line: k values assessed by electrolyte leakage; dashed line: proportion of seedlings survival (PSS) assessed by visual inspection.

Statistical analysis

Frost hardiness of the different development stages described by the LT50-values was related to the species macroclimatic niches. Therefore, Therefore, global occurrence data on the investigated species, based on floristic atlases and online published databases, were georeferenced and digitized using ArcMap (ESRI). Climatic data were extracted from the Worldclim dataset ([15]) in a 2.5 arc minutes grid across the whole distribution range. For the following analyses we used the lower 1st percentile of all macroclimatic variables that described frost conditions (Tab. 2). To test the first hypothesis, i.e., the relationship between frost tolerance and the climatic conditions in winter, linear regression models were applied using LT50-values and the minimum temperature of the coldest month, as well as the minimum temperatures of each months from November to May. As for the second hypothesis, differences between adults and seedlings and between different seedling ages were verified using paired t-tests. Additionally, we related the LT50-values of the adult individuals to the LT50-values of all three development stages of the seedlings, using linear regression models. Differences in seedlings frost tolerance among species was tested using a generalized linear model with binomial error distribution, including temperature level and species identity as fixed factors. These two-factorial models were subjected to Tukey’s post-hoc tests to determine which species differed from each other. Similarly, we tested for differences in frost tolerance among the different seedlings development stages, using the temperature level and the seedling age as fixed factors.

Tab. 2 - Minimum temperature of the coldest month (the lower 1st percentile - °C) and frost tolerance expressed as LT50-values (°C) of the seedlings (one-week, two-weeks and four-weeks old) and the adult individuals for all the eight species studied. Different letters in the seedlings columns indicate statistically significant differences between species according to Tukey’s post-hoc tests.

The sigmoid regressions were calculated with Sigmaplot 11.0 (Systat Software®, v. 2008), whereas all the other statistical analyses were carried out using the software package R version 2.12.0 ([30]).

Results 

Frost hardiness of the adult individuals, expressed as LT50-values, ranged from -45.29 (T. cordata) to -22.55 °C (A. glutinosa - Tab. 2). No significant relationships were found between the LT50 value of the adult individuals and any of the macroclimatic variables tested (Tab. 3, Fig. 3). Moreover, no significant correlations were detected between the frost hardiness of seedlings and any climatic variable (Tab. 4). There was one marginally significant effect between frost hardiness of four-weeks-old seedlings and the minimum temperature of April, but the direction of the effect was opposite to the expectation, with the tendency of species with lower frost hardiness of seedlings to occur at the higher minimum temperature. In addition, no significant correlations were found between the frost hardiness of any development stage of the seedlings and the frost hardiness of the adult individuals (Fig. 4).

Tab. 3 - Summary of R² and p-values of the linear regression models tested for relationships between LT50-values of the buds of adult individuals and the macroclimatic variables as drawn from the Worldclim dataset. For all regressions, degrees of freedom = 6.
Fig. 3 - Correlation between frost hardiness of adult individuals expressed as LT50-values and minimum temperature of the coldest month. (Ag): Alnus glutinosa; (An): Acer negundo; (Ap): Acer platanoides; (Bp): Betula pendula; (Fs): Fagus sylvatica; (Pa): Picea abies; (Pm): Pseudotsuga menziesii; (Tc): Tilia cordata.
Tab. 4 - Summary of R² and p-values of the linear regression models tested for relationships between LT50-values of all three development stages of the seedlings (one-week, two-weeks and four-weeks old), as obtained from the 4-parametric sigmoid regression, and LT50-values of the adult individuals, as well as the macroclimatic variables (from the Worldclim dataset). For all regressions, degrees of freedom = 6.
Fig. 4 - Frost hardiness of one-week (A), two-weeks (B) as well as four-weeks old seedlings (C) and frost hardiness of the adult individuals expressed as LT50-values. (Ag): Alnus glutinosa; (An) Acer negundo; (Ap): Acer platanoides; (Bp): Betula pendula; (Fs): Fagus sylvatica; (Pa): Picea abies; (Pm): Pseudotsuga menziesii; (Tc): Tilia cordata.

Frost hardiness of the seedlings ranged from -12.08 to -6.01 °C, whereby A. glutinosa and T. cordata exhibited the lowest frost hardiness (one-week old seedlings) and P. menziesii (two-weeks old seedlings) showed the highest (Tab. 2). Species only differed in the age class of one-week and two-weeks old seedlings, while there was no statistically significant difference in frost tolerance among species in four-weeks old seedlings. One-week and four-week old seedlings of T. cordata and A. glutinosa were significantly more sensitive to frost than those of F. sylvatica, P. abies and P. menziesii (Tab. 2). Across all species, frost hardiness of seedlings was significantly lower than that of adults (p < 0.001 for adults versus one-, two- and four-weeks old seedlings after paired t-tests). While frost tolerance across all species differed between one- and two-weeks old seedlings (p = 0.0028), there was no difference between two- and four-weeks old seedlings (p = 0.883), according to paired t-tests.

Seedlings of A. negundo, A. platanoides, A. glutinosa, F. sylvatica and T. cordata showed an increase in frost hardiness with increasing age with a maximal difference of 3.9 °C between the one-week-old and the four-weeks-old developmental stage (T. cordata - Tab. 2). In contrast, the seedlings of B. pendula, P. abies and P. menziesii had the highest frost hardiness at the two-weeks-old developmental stage.

Discussion 

We found no relationship between the frost tolerance of the eight tree species and the macroclimatic variables derived from their geographical distribution range, neither for adult adults winter buds or needles, nor for seedlings at three different ages. Thus, the hypothesis of a relationship between species’ frost hardiness and the extension of their distribution range to regions with low winter temperatures (our first hypothesis) has to be rejected. As this result is exactly the opposite of the current biogeographical understanding, we have to consider that there might be several methodological reasons. A major drawback of the approach adopted in this study was that all plant material subjected to frost hardiness measurements was taken from the same geographical location, i.e., Halle (Saale). This may preclude the possibility of detecting any effects of intra-specific adaptation. Indeed, it might be that the study species were adapted to the much more moderate frost conditions at the study site than to those encountered at the geographical distribution limits, where the climatic variables considered had been drawn from. Even the lowest measured frost hardiness of any adult tree (i.e., A. glutinosa with a frost hardiness of -22.6 °C) was high enough to safely survive under the climatic winter conditions in the study region. Many studies has investigated local adaptation to frost, demonstrating a relationship between frost hardiness and the climatic conditions of the populations’ geographic origins, with species from northern provenances or higher elevation being more frost tolerant than species from southern provenances or lower elevation ([6], [20], [1], [21], [23]). In particular, species with a wide distribution range such as Quercus robur L. have developed ecotypes with respect to frost hardiness ([13]). However, if intra-specific adaptation had blurred inter-specific differences in frost tolerance between species, this would indicate that intra-specific variation in frost hardiness might be higher than variation among species growing in the same area. Testing this assumption would require to take samples of both adults and seedlings from different climatic zones for different species. Preferably, the different provenances would also include the range boundaries of the different species. For adult trees, there are not only logistic obstacles with such a sampling scheme, as simultaneous sampling from different regions involves the risk to include plants with a different degree of acclimatization. It is well known that winter frost hardiness is acquired gradually and the degree of hardening depends on outdoor temperatures ([33], [5]). While we could control for differences in the degree of hardening by taking all bud and needle samples of adult trees at the time of maximum hardening, this will probably not be possible when sampling over a large geographical area. A solution to this challenge would be provenance trials of different tree species grown under the same common garden conditions. For seedlings, simultaneous testing of provenances is simpler as the seeds can be germinated at the same time.

Our study is not the only one that failed to detect differences in frost tolerance between different species. Comparing eight congeneric pairs of grassland species with contrasting distribution ranges, Hofmann et al. ([16]) also failed to confirm the correspondence between frost resistance and macroclimatic niche variables. Similar to our results, Baltzer et al. ([4]) detected no relationship between the fundamental and realized niches with respect to the seasonality of the rainfall for different tropical tree species.

In contrast to our expectation, seedlings frost tolerance did not reflect the species’ macroclimatic niche better than that of adults. Since long, frost hardiness of seedlings has been claimed to be an important limiting factor of the distribution ranges of tree species. This statement is mainly based on cursory observations on lethal effects of exceptional frost events on seedlings, as reported by Szafer ([35]) for F. sylvatica. Our results did reveal only moderate differences in frost hardiness of seedlings of the different species studied. Thus, seedlings of different tree species would be affected quite similarly by exceptional late frost events. As a consequence, seedling frost hardiness can hardly be a good predictor for the different distribution ranges of tree species. Similar doubts on the relevance of late frost events have also been expressed by other authors, such as by Funkenberg et al. ([14]) for the establishment of F. japonica seedlings.

It has to be considered that our study focused only on seedling frost lethality and not on the effects of non-lethal freezing temperatures on seedling performance. It is possible, that non-lethal freezing temperatures higher than the measured LT50-values result in a weakening of plants, making them more vulnerable to subsequent frost events or to other types of biotic or abiotic stress ([32]). For example, these non-lethal effects led to a reduced growth of Q. robur L. seedlings and the ensuing growth reduction has been interpreted as a limiting factor for the northern distribution boundary of this species ([32]). Similarly, comparing the growing rates of F. sylvatica seedlings, Kreyling et al. ([23]) reported a reduced growth as a consequence of spring frost events in the Bulgarian but not in the German provenance. Non-lethal freezing temperatures also resulted in reduced growth and survival of Digitalis purpurea L. seedlings at the altitudinal distribution limit ([9]).

We found support for our second hypothesis as the investigated seedlings showed increasing frost hardiness with increasing age for five of eight species. With the exception of the two evergreen species, P. abies and P. menziesii, the four-weeks old seedling were more frost tolerant than the one-week old seedlings. This might point to fundamental differences in the sensitivity of cotyledons and primary leaves between angiosperm and gymnosperm seedlings. While cotyledons seem to be more susceptible to frosts than primary leaves in angiosperms, while the opposite might be the case for gymnosperms. However, organ-specific tests would be required to test this hypothesis.

As frost events become rarer with the progress of the growing season and frost hardiness of our six angiosperm seedlings increases with seedling age, the risk of being damaged by late frost events in spring decreases disproportionally with time. This underlines the role of the germination timing. A later germination has been often interpreted as acclimation to late frost events. For example, Chuine & Cour ([11]) postulated that the timing of germination is adaptive to climate conditions of the species’ geographical distribution range. Our results show that this might not be the case, as frost hardiness of one-week old seedlings of different species is rather similar, which has the consequence that early germinating tree species take higher risks, irrespective of their macroclimatic niche.

Conclusion 

Based on our experimental results, predictions on the geographical distribution range of the eight species considered based on their frost tolerance seems to be unfeasible. Thus, the general assumption that minimum temperatures below a species-specific threshold simply kill needles, buds or seedlings has to be reconsidered. Nevertheless, frost might operate through a multitude of other mechanisms, affecting organs different from needles or buds, or biotic interactions such as pollination, thus contributing to limit the distribution ranges of European tree species.

Acknowledgments 

We thank C. Voigt for the useful comments on seedlings cultivation and G. Seidler for the help with compiling the distribution data and maps. This study was financially supported by the graduate scholarship of Saxony- Anhalt.

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Hofmann M, Jager M, Bruelheide H (2014).
Relationship between frost hardiness of adults and seedlings of different tree species
iForest - Biogeosciences and Forestry 7: 282-288. - doi: 10.3832/ifor1076-007
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Paper ID# ifor1076-007
Title Relationship between frost hardiness of adults and seedlings of different tree species
Authors Hofmann M, Jager M, Bruelheide H
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